Department of Statistics, University of Delhi, New Delhi 110007, India.
Department of Biochemistry, Institute of Home Economics, University of Delhi, New Delhi 110016, India.
Clin Chim Acta. 2018 Jul;482:136-143. doi: 10.1016/j.cca.2018.03.041. Epub 2018 Apr 6.
Whereas many previous studies have revealed that mitochondrial DNA (mtDNA) polymorphism T16189C is associated with the risk of cancer and Type 2 diabetes mellitus (T2DM), there are others that have disputed the same. As a result, clarity on the role of mitochondrial T16189C in these disorders is missing. The aim of this study is to evaluate the association of T16189C polymorphism with the risk of cancer and T2DM development by pooling all case-control studies available.
Published studies till November 2017 were searched from PubMed, Google scholar, Google and EMBASE and isolated a total of 36 studies having 44,203 subjects (20,439 cases and 23,764 controls) based on strict inclusion and exclusion criteria. We used the statistical software "R" to calculate the Pooled Odds Ratios and 95% confidence intervals to evaluate the association of T16189C polymorphism with a possible risk towards cancer and T2DM development.
From the meta-analysis, we obtained Pooled Odds Ratios using Random effect model for cancer (OR: 1.20, 95% CI: 0.96-1.49, P = 0.104) and for T2DM (OR: 1.22, 95% CI: 1.09-1.36, P = 0.0004). In the subgroup analysis with Random effect model, we found that both Asians and Caucasians were at a statistically significant risk (OR: 1.25, P < 0.0001 and OR: 1.20, P < 0.0001, respectively) for the development of T2DM, whereas, a statistically non-significant risk (OR: 1.28 P = 0.1965 and OR: 1.16, P = 0.1148) emerged for the development of cancer. There was no evidence of a significant publication bias (Egger's and Begg's test) in this meta-analysis. Further sensitivity analysis also demonstrated that our meta-analysis was relatively stable and credible.
Individuals with 'C' allele at position 16,189 within the mitochondrial D-loop are seemingly at a higher risk of developing T2DM and cancer. However, before arriving at generalizations, it would be pertinent to conduct similar studies in different populations with larger numbers to corroborate these results, especially in cancer.
虽然许多先前的研究表明线粒体 DNA（mtDNA）多态性 T16189C 与癌症和 2 型糖尿病（T2DM）的风险相关，但也有其他研究对此提出质疑。因此，线粒体 T16189C 在这些疾病中的作用尚不清楚。本研究旨在通过合并所有可用的病例对照研究来评估 T16189C 多态性与癌症和 T2DM 发病风险的关系。
从 PubMed、Google Scholar、Google 和 EMBASE 检索截至 2017 年 11 月发表的研究，根据严格的纳入和排除标准，共筛选出 36 项研究，共纳入 44203 例受试者（20439 例病例和 23764 例对照）。我们使用统计软件“R”计算汇总优势比和 95%置信区间，以评估 T16189C 多态性与癌症和 T2DM 发病风险的关系。
通过荟萃分析，我们使用随机效应模型得出癌症的汇总优势比（OR：1.20，95%CI：0.96-1.49，P=0.104）和 T2DM（OR：1.22，95%CI：1.09-1.36，P=0.0004）。在随机效应模型的亚组分析中，我们发现亚洲人和高加索人患 T2DM 的风险均具有统计学意义（OR：1.25，P<0.0001 和 OR：1.20，P<0.0001），而患癌症的风险无统计学意义（OR：1.28，P=0.1965 和 OR：1.16，P=0.1148）。本荟萃分析无明显发表偏倚（Egger 和 Begg 检验）。进一步的敏感性分析也表明，我们的荟萃分析相对稳定且可信。
线粒体 D 环 16189 位 C 等位基因的个体似乎更容易患 T2DM 和癌症。然而，在得出一般性结论之前，有必要在不同人群中进行类似的研究，以更大的样本量来证实这些结果，特别是在癌症方面。
