160YPVVKKPKLTEE171 中的 164K、165K 和 167K 残基是鹅细小病毒 VP1 核输入所必需的。
The 164 K, 165 K and 167 K residues in 160YPVVKKPKLTEE171 are required for the nuclear import of goose parvovirus VP1.
机构信息
Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang District, Chengdu City, Sichuan Province 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang District, Chengdu City, Sichuan Province 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Wenjiang District, Chengdu City, Sichuan Province 611130, China.
Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang District, Chengdu City, Sichuan Province 611130, China.
出版信息
Virology. 2018 Jun;519:17-22. doi: 10.1016/j.virol.2018.03.020. Epub 2018 Apr 6.
goose parvovirus (GPV) belongs to the Dependoparvovirus genus in Parvovirinae subfamily within Parvoviridae family, is the etiological agent of Derzsy's disease. Nuclear localization signal (NLS) is important for parvovirus lifecycle in the delivery of genomes and the structural protein of progeny virus into the nucleus. Here, NLS was first identified in GPV. By using the PSORT II program, a basic region (BR, 160YPVVKKPKLTEE171) in the N-terminus of VP1 was found, which predicted as putative NLS motif of goose parvovirus capsid. The GPV BR could transfer both small reporter proteins (EGFP) and large reporter protein (β-galactosidase) into the nucleus by Immunofluorescence assay. Furthermore, the K164A, or K165A, or K167A substitutions mutation of GPV VP1 did abolish its nuclear localization, suggesting that the 164 K, 165 K and 167 K residues in the 160YPVVKKPKLTEE171 are required for its for nuclear import. Our finding may help us to gain a better understand of GPV lifecycle.
鹅细小病毒（GPV）属于细小病毒科依赖病毒属细小病毒亚科，是 Derzsy 病的病原体。核定位信号（NLS）对于细小病毒的基因组传递和新生病毒的结构蛋白进入细胞核的生命周期非常重要。在这里，首次在 GPV 中鉴定出 NLS。通过使用 PSORT II 程序，在 VP1 的 N 端发现了一个碱性区域（BR，160YPVVKKPKLTEE171），该区域被预测为鹅细小病毒衣壳的潜在 NLS 基序。GPV BR 可以通过免疫荧光测定将小报告蛋白（EGFP）和大报告蛋白（β-半乳糖苷酶）都转位到细胞核中。此外，GPV VP1 的 K164A、或 K165A、或 K167A 取代突变会使其失去核定位，这表明 160YPVVKKPKLTEE171 中的 164 K、165 K 和 167 K 残基对于其核输入是必需的。我们的发现可能有助于更好地了解 GPV 的生命周期。
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