Microbiology Laboratory, Department of Botany, The University of Burdwan, 713104, West Bengal, India.
Comput Biol Chem. 2018 Jun;74:190-200. doi: 10.1016/j.compbiolchem.2018.03.018. Epub 2018 Mar 19.
Lipase (triacylglycerol acylhydrolase, EC 3.1.1.3) catalyzes tri-, di-, and monoacyl glycerol of fat into glycerol and fatty acids. It has important roles in the digestion of lipids in living organisms and industrially as laundry detergents along with proteases. The microbial lipases are more stable, active and economically feasible compared to plant and animal sources. Hence, much attention was given to the maximum production of the enzyme from the microbial sources. The phylogenetic analysis revealed that the amino acid sequence of lipase protein and their corresponding cDNA of Pseudomonas aeruginosa clustered with Pseudomonas stutzeri among different species of Pseudomonas, while P. aeruginosa PA1 clustered with P. aeruginosa SJTD-1 among different strains of P. aeruginosa. The lipase of P. aeruginosa PA1 was a monomeric, acidic and thermostable protein having a molecular weight ranging in between 32.72 to 34.89 kDa. The protein was abundant with random coils and alpha helices in its secondary structure. The tertiary model showed 96.310 score as an overall quality factor. Hence, this in silico study gives some useful information about the lipase protein without performing crystal structure assessment by X-ray Crystallography or NMR study in wet lab experiments which could be helpful for isolation and characterization of the enzyme in vitro.
脂肪酶（三酰基甘油酰基水解酶，EC 3.1.1.3）催化脂肪中的三酰基、二酰基和单酰基甘油生成甘油和脂肪酸。它在生物体内脂质的消化和工业上作为洗衣洗涤剂与蛋白酶一起具有重要作用。与植物和动物来源相比，微生物脂肪酶更稳定、更活跃、更经济可行。因此，人们非常关注从微生物来源中最大限度地生产这种酶。系统发育分析表明，铜绿假单胞菌脂肪酶蛋白的氨基酸序列及其相应的 cDNA 与假单胞菌属中不同种的施氏假单胞菌聚类，而铜绿假单胞菌 PA1 与不同株的铜绿假单胞菌 SJTD-1 聚类。铜绿假单胞菌 PA1 的脂肪酶是一种单体、酸性和热稳定的蛋白质，分子量在 32.72 到 34.89 kDa 之间。该蛋白质的二级结构中富含无规则卷曲和α螺旋。三级模型的总质量因子得分为 96.310。因此，这项计算机研究提供了一些关于脂肪酶蛋白的有用信息，而无需在湿实验室实验中进行 X 射线晶体学或 NMR 研究来评估晶体结构，这对于在体外分离和表征该酶可能会有所帮助。
