School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle and Hunter Medical Research Institute, Newcastle, New South Wales 2308, Australia; School of Medicine, Faculty of Health, University of Tasmania, Hobart, Tasmania 7000, Australia.
School of Medicine, Faculty of Health, University of Tasmania, Hobart, Tasmania 7000, Australia.
Environ Res. 2018 Jul;164:625-635. doi: 10.1016/j.envres.2018.03.029. Epub 2018 Apr 5.
In utero exposure to particulate matter (PM) from a range of sources is associated with adverse post-natal health; however, the effect of maternal exposure to community-sampled PM on early post-natal lung and immune development is poorly understood.
Using a mouse model, we aimed to determine whether in utero exposure to PM alters early post-natal lung function and immune cell populations. We used PM collected from ceiling voids in suburban houses as a proxy for community PM exposure.
Pregnant C57BL/6 mice were intranasally exposed to ceiling derived PM, or saline alone, at gestational day (E) 13.5, 15.5, and 17.5. When mice were two weeks old, we assessed lung function by the forced oscillation technique, and enumerated T and B cell populations in the spleen and thymus by flow cytometry.
Maternal exposure to PM impaired somatic growth of male offspring resulting in reduced lung volume and deficits in lung function. There was no effect on thymic T cell populations in dams and their male offspring but PM decreased the CD4 +CD25 + T cell population in the female offspring. In contrast, maternal exposure to PM increased splenic CD3 +CD4 + and CD3 +CD8 + T cells in dams, and there was some evidence to suggest inhibition of splenic T cell maturation in male but not female offspring.
Our findings suggested that maternal exposure to ceiling void PM has the capacity to impair early somatic growth and alter early life immune development in a sex specific manner.
胎儿期暴露于多种来源的颗粒物（PM）与产后健康不良有关；然而，母体暴露于社区采集的 PM 对产后早期肺和免疫发育的影响知之甚少。
本研究使用小鼠模型，旨在确定胎儿期暴露于 PM 是否会改变产后早期肺功能和免疫细胞群。我们使用从郊区房屋天花板空隙中采集的 PM 作为社区 PM 暴露的替代物。
在妊娠第 13.5、15.5 和 17.5 天，将怀孕的 C57BL/6 小鼠经鼻腔暴露于天花板衍生的 PM 或单独生理盐水。当小鼠两周大时，我们通过强迫振荡技术评估肺功能，并通过流式细胞术计数脾和胸腺中的 T 和 B 细胞群。
母体暴露于 PM 会损害雄性后代的体生长，导致肺容积减少和肺功能缺陷。对母体和雄性后代的胸腺 T 细胞群没有影响，但 PM 降低了雌性后代的 CD4+CD25+T 细胞群。相比之下，母体暴露于 PM 增加了母体和雄性后代的脾 CD3+CD4+和 CD3+CD8+T 细胞，但有一些证据表明，PM 抑制了雄性而非雌性后代的脾 T 细胞成熟。
我们的研究结果表明，母体暴露于天花板空隙 PM 有能力以性别特异性的方式损害早期的体生长和改变早期生命的免疫发育。
