Song Yong, Chen Ling, Bennett Ellen, Wheeler Amanda J, Southam Katherine, Yen Seiha, Johnston Fay, Zosky Graeme R
Menzies Institute for Medical Research, College of Health and Medicine, University of Tasmania, Hobart, TAS 7001, Australia.
School of Biomedical Sciences and Pharmacy, University of Newcastle, Callaghan, NSW 2308, Australia.
Toxics. 2023 Jan 9;11(1):61. doi: 10.3390/toxics11010061.
Emerging evidence suggests that inhalation of particulate matter (PM) can have direct adverse effects on liver function. Early life is a time of particular vulnerability to the effects of air pollution. On that basis, we tested whether in utero exposure to residential PM has an impact on the developing liver. Pregnant mice (C57BL/6J) were intranasally administered 100 µg of PM sampled from residential roof spaces (~5 mg/kg) on gestational days 13.5, 15.5, and 17.5. The pups were euthanized at two weeks of age, and liver tissue was collected to analyse hepatic metabolism (glycogen storage and lipid level), cellular responses (oxidative stress, inflammation, and fibrosis), and genotoxicity using a range of biochemical assays, histological staining, ELISA, and qPCR. We did not observe pronounced effects of environmentally sampled PM on the developing liver when examining hepatic metabolism and cellular response. However, we did find evidence of liver genomic DNA damage in response to in utero exposure to PM. This effect varied depending on the PM sample. These data suggest that in utero exposure to real-world PM during mid-late pregnancy has limited impacts on post-natal liver development.
新出现的证据表明，吸入颗粒物（PM）会对肝功能产生直接不利影响。生命早期是特别容易受到空气污染影响的时期。在此基础上，我们测试了子宫内暴露于住宅PM是否会对发育中的肝脏产生影响。在妊娠第13.5、15.5和17.5天，对怀孕小鼠（C57BL/6J）经鼻给予100μg从住宅屋顶空间采集的PM（约5mg/kg）。幼崽在两周龄时安乐死，并收集肝脏组织，使用一系列生化分析、组织学染色、ELISA和qPCR分析肝脏代谢（糖原储存和脂质水平）、细胞反应（氧化应激、炎症和纤维化）以及基因毒性。在检查肝脏代谢和细胞反应时，我们未观察到环境采样的PM对发育中的肝脏有明显影响。然而，我们确实发现了子宫内暴露于PM后肝脏基因组DNA损伤的证据。这种影响因PM样本而异。这些数据表明，妊娠中后期子宫内暴露于实际环境中的PM对出生后肝脏发育的影响有限。
