Nouha Klai, Kumar Ram Saurabh, Balasubramanian Sellamuthu, Tyagi Rajeshwar Dayal
Université du Québec, Institut national de la Recherche Scientifique, Centre Eau, Terre & Environnement, 490 de la Couronne, Québec G1K 9A9, Canada.
Université du Québec, Institut national de la Recherche Scientifique, Centre Eau, Terre & Environnement, 490 de la Couronne, Québec G1K 9A9, Canada.
J Environ Sci (China). 2018 Apr;66:225-245. doi: 10.1016/j.jes.2017.05.020. Epub 2017 May 22.
Extracellular polymeric substances (EPS) produced by microorganisms represent biological macromolecules with unfathomable potentials and they are required to be explored further for their potential application as a bioflocculant in various wastewater sludge treatment. Although several studies already exist on biosynthetic pathways of different classical biopolymers like alginate and xanthan, no dedicated studies are available for EPS in sludge. This review highlights the EPS composition, functionality, and biodegradability for its potential use as a carbon source for production of other metabolites. Furthermore, the effect of various extraction methods (physical and chemical) on compositional, structural, physical and functional properties of microbial EPS has been addressed. The vital knowledge of the effect of extraction method on various important attributes of EPS can help to choose the suitable extraction method depending upon the intended use of EPS. The possible use of different molecular biological techniques for enhanced production of desired EPS was summarized.
微生物产生的胞外聚合物（EPS）是具有巨大潜力的生物大分子，作为生物絮凝剂在各种废水污泥处理中的潜在应用有待进一步探索。尽管已经有多项关于藻酸盐和黄原胶等不同传统生物聚合物生物合成途径的研究，但尚未有针对污泥中EPS的专门研究。本综述着重介绍了EPS的组成、功能和生物降解性，以探讨其作为生产其他代谢物的碳源的潜在用途。此外，还讨论了各种提取方法（物理和化学方法）对微生物EPS的组成、结构、物理和功能特性的影响。了解提取方法对EPS各种重要特性的影响，有助于根据EPS的预期用途选择合适的提取方法。本文还总结了利用不同分子生物学技术提高所需EPS产量的可能性。
