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[从阿比让(科特迪瓦)市售阿蒂克中鉴定酵母:初步研究]

[Identification of yeasts from commercial attiéké in Abidjan (Côte d'Ivoire): Preliminary study].

作者信息

Kouadio-Yapo C G, Dou G S P, Aka N A D, Zika K D, Adoubryn K D, Dosso M

机构信息

Laboratoire de parasitologie-mycologie, UFR sciences médicales, 01 BP 166, Abidjan, Côte d'Ivoire.

Laboratoire de parasitologie-mycologie, UFR sciences médicales, 01 BP 166, Abidjan, Côte d'Ivoire.

出版信息

J Mycol Med. 2018 Jun;28(2):305-309. doi: 10.1016/j.mycmed.2018.03.006. Epub 2018 Apr 6.

Abstract

OBJECTIVE

This study aims to identify yeasts from commercial attiéké.

PATIENTS AND METHODS

This descriptive cross-sectional survey involving 103 samples of attiéké sold on the market from August to September 2017. It was conducted from the mycology laboratory of the Institut Pasteur of Côte d'Ivoire. The samples were seeded on YGC medium. The colonies of positive culture were counted and seeded again on chromogen medium. Identification of yeasts species was carried out by MALDI-TOF mass spectrometry (Vitek MS bioMérieux).

RESULTS

As a whole, 63 samples were contaminated with fungi i.e. an overall prevalence of 61.1 %. Fifty-nine of yeasts were identified i.e. 57.2 %. Candida rugosa was most frequently isolated (62.7 %), followed by Ccandida albicans (8.5 %), Candida krusei (8.5 %) et Candida tropicalis (8.5 %). Geotrichum candidum represented 6.8 % isolated yeasts. The yeast average load is 10,124×10 CFU/g with high contamination of commercial attiéké in the commune of Abobo.

CONCLUSION

The contamination of attiéké by yeasts is a reality. C. rugosa was most frequently isolated. It would be necessary to investigate the food chain to determine the source of contamination.

摘要

目的

本研究旨在从市售阿蒂克(一种发酵木薯粉)中鉴定酵母菌。

患者与方法

这是一项描述性横断面调查,涉及2017年8月至9月在市场上销售的103份阿蒂克样本。该调查在科特迪瓦尔多尔巴斯德研究所的真菌学实验室进行。样本接种于YGC培养基上。对阳性培养菌落进行计数,并再次接种于显色培养基上。通过基质辅助激光解吸电离飞行时间质谱(Vitek MS bioMérieux)对酵母菌种进行鉴定。

结果

总体而言,63份样本被真菌污染,即总体患病率为61.1%。鉴定出59种酵母菌,即占57.2%。皱落假丝酵母分离频率最高(62.7%),其次是白色念珠菌(8.5%)、克鲁斯假丝酵母(8.5%)和热带假丝酵母(8.5%)。白地霉占分离出酵母菌的6.8%。酵母平均载量为10,124×10 CFU/g,阿博博公社的市售阿蒂克污染程度较高。

结论

阿蒂克被酵母菌污染是事实。皱落假丝酵母分离频率最高。有必要对食物链进行调查以确定污染源。

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