雌二醇刺激子宫质膜蛋白酶激活剂。

Estradiol stimulates a uterine plasma membrane protease activator.

作者信息

Jazín E E, Dickerman H W, Henrikson K P

机构信息

Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201.

出版信息

Endocrinology. 1988 Feb;122(2):500-3. doi: 10.1210/endo-122-2-500.

DOI:10.1210/endo-122-2-500

PMID:2962850

Abstract

An estrogen-regulated arginine esteropeptidase is present in the immature rat uterus. The enzymatic complex consists of a membrane-bound activator and a soluble proenzyme. The activator is under strong estrogen control; its activity increases 10-fold 3 h after a single dose of 17 beta-estradiol. The subcellular localization of the activator is determined by a radioactive assay of fractions prepared by sucrose density centrifugation. The distribution of activity parallels the distribution of two plasma membrane markers, Mg2+-ATPase and 5'-nucleotidase. Electron micrographic visualization of the gradient fractions containing the activator reveals a population of vesicles 0.2-0.5 micron in diameter.

摘要

一种雌激素调节的精氨酸酯肽酶存在于未成熟大鼠子宫中。该酶复合物由一个膜结合激活剂和一个可溶性酶原组成。激活剂受雌激素的严格控制；单次给予17β-雌二醇3小时后，其活性增加10倍。通过蔗糖密度离心制备的各组分的放射性测定来确定激活剂的亚细胞定位。活性分布与两种质膜标志物Mg2 + -ATP酶和5'-核苷酸酶的分布平行。含有激活剂的梯度组分的电子显微镜观察显示一群直径为0.2 - 0.5微米的小泡。