雌激素诱导的子宫过氧化物酶的亚细胞定位
Subcellular localization of oestrogen-induced uterine peroxidase.
作者信息
Lyttle C R, Jellinck P H
出版信息
Biochem J. 1976 Nov 15;160(2):237-41. doi: 10.1042/bj1600237.
The distribution of oestrogen-induced peroxidase in the resuspended 8000g pellet of rat uterine homogenates was examined by centrifugation in a sucrose density gradient. Within 10h of treatment with oestradiol, peroxidase activity was found in a region devoid of catalase or urate oxidase (peroxisomal markers) which did not overlap the fractions containing succinate dehydrogenase (mitochondrial marker) or acid phosphatase (lysosomal marker). The induced uterine enzyme was localized in reticular membrane-bound vesicles with isopycnic density of 1.28g/ml from which it could be released by treatment with detergent.
通过在蔗糖密度梯度中离心，检测了雌激素诱导的过氧化物酶在重悬的大鼠子宫匀浆8000g沉淀中的分布。在用雌二醇处理10小时内，在不含过氧化氢酶或尿酸氧化酶（过氧化物酶体标记物）的区域发现了过氧化物酶活性，该区域与含有琥珀酸脱氢酶（线粒体标记物）或酸性磷酸酶（溶酶体标记物）的组分不重叠。诱导的子宫酶定位于等密度为1.28g/ml的网状膜结合小泡中，用去污剂处理可从中释放出来。
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