Eljaszewicz Andrzej, Jankowski Michal, Wiese-Szadkowska Malgorzata, Gackowska Lidia, Michalkiewicz Jacek, Zegarski Wojciech, Moniuszko Marcin
Department of Regenerative Medicine and Immune Regulation, Medical University of Bialystok, Poland.
Department of Surgical Oncology, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University of Torun, Poland.
Contemp Oncol (Pozn). 2018 Mar;22(1A):33-37. doi: 10.5114/wo.2018.73881. Epub 2018 Mar 5.
Immune responses within the tumor depend on the ability of leukocytes to migrate from peripheral circulation into the local microenvironment. This process is controlled by mechanisms that guide leukocytes to the side of inflammation, allowing them to cross vascular endothelial barrier. Monocytes/macrophages are the predominant population of leukocyte infiltrate of many tumors, including, gastric cancer. However, to date mechanisms that control monocyte trafficking to the side of tumor growth are not fully elucidated.
It this study we aimed to evaluate transmigratory potential of peripheral blood monocytes from gastric cancer patients.
By using multicolor flow cytometry we assessed expression of β1- and β2-integrins on peripheral blood monocytes from gastric cancer patients.
We found increased frequencies of VLA-4 and VLA-6 expressing monocytes and increased expression of analyzed β2-integrins in gastric cancer patients when compared to age matched controls.
In summary, this study revealed that gastric cancer increases transmigratory potential of peripheral blood monocytes.
肿瘤内的免疫反应取决于白细胞从外周循环迁移至局部微环境的能力。这一过程受多种机制控制，这些机制引导白细胞至炎症部位，使其能够穿越血管内皮屏障。单核细胞/巨噬细胞是包括胃癌在内的许多肿瘤中白细胞浸润的主要群体。然而，迄今为止，控制单核细胞向肿瘤生长部位迁移的机制尚未完全阐明。
本研究旨在评估胃癌患者外周血单核细胞的迁移潜力。
通过多色流式细胞术，我们评估了胃癌患者外周血单核细胞上β1和β2整合素的表达。
与年龄匹配的对照组相比，我们发现胃癌患者中表达VLA-4和VLA-6的单核细胞频率增加，且所分析的β₂整合素表达增加。
总之，本研究表明胃癌会增加外周血单核细胞的迁移潜力。
