Melgunov V I, Jindal S, Belikova M P
Department of Molecular Biology, School of Biology, M.V. Lomonosov State University, Moscow, USSR.
FEBS Lett. 1988 Jan 25;227(2):157-60. doi: 10.1016/0014-5793(88)80888-3.
The effect of 10 low molecular mass alkanols on the activity of Ca-ATPase (EC 3.6.1.38), Ca uptake and Ca efflux as well as the functional efficiency of the Ca pump in the fragmented sarcoplasmic reticulum of rabbit skeletal muscles has been studied. Some alkanols, especially when taken at low concentration, have been found to stimulate the activity of the Ca pump and Ca-ATPase, namely tert-butanol, isopropanol and ethanol (from the group of hydrophilic alkanols), and pentanol, isopentanol and hexanol (from the more hydrophobic alkanols). Methanol (from the first group) and isobutanol, butanol and propanol (from the second) do not stimulate the Ca pump compared with the control. The specific effect of different alkanols cannot be explained in terms of a unitary mechanism based on 'fluidity' changes of the membrane. It is assumed that, at low concentrations, certain alkanols (or groups of related alkanols) are able to promote the specific transition of membrane proteins into the active state, whereas at higher concentrations all alkanols provide for the non-functional state of the proteins.
研究了10种低分子量链烷醇对兔骨骼肌肌浆网碎片中Ca - ATP酶（EC 3.6.1.38）活性、Ca摄取和Ca流出以及Ca泵功能效率的影响。已发现某些链烷醇，特别是在低浓度时，能刺激Ca泵和Ca - ATP酶的活性，即叔丁醇、异丙醇和乙醇（来自亲水性链烷醇组），以及戊醇、异戊醇和己醇（来自疏水性更强的链烷醇组）。与对照组相比，甲醇（来自第一组）以及异丁醇、丁醇和丙醇（来自第二组）不会刺激Ca泵。不同链烷醇的特定作用无法用基于膜“流动性”变化的单一机制来解释。据推测，在低浓度时，某些链烷醇（或相关链烷醇组）能够促进膜蛋白向活性状态的特定转变，而在较高浓度时，所有链烷醇都会使蛋白处于无功能状态。
