Zeller R, Bloch K D, Williams B S, Arceci R J, Seidman C E
Department of Genetics, Harvard Medical School, Boston, Massachusetts.
Genes Dev. 1987 Sep;1(7):693-8. doi: 10.1101/gad.1.7.693.
Expression of the gene encoding atrial natriuretic factor (ANF) during mouse cardiac embryogenesis was studied using in situ hybridization to histologic sections. ANF mRNA was first detected in a subpopulation of myocardial cells at day 8 of embryogenesis. During day 9, abundant hybridization of the ANF probe to the atrium and the primitive ventricle was found. At day 14, strong labeling of cells in both atria and the trabeculated regions of the left ventricle was seen, whereas fewer cells in the right ventricle expressed the ANF gene. The developmental regulation of ANF gene expression in atrial and ventricular cells suggests an important role for this peptide hormone in the embryo. Localized expression of the ANF gene provides a marker for discerning subpopulations of cardiac cells during embryogenesis. Identification of embryonic ventricular ANF mRNA transcripts may have implications with respect to pathologic reexpression of the ANF gene in adult ventricles.
利用组织学切片原位杂交技术，研究了小鼠心脏胚胎发育过程中编码心房利钠因子（ANF）的基因表达情况。在胚胎发育第8天，首次在心肌细胞亚群中检测到ANF mRNA。在第9天，发现ANF探针与心房和原始心室有大量杂交。在第14天，可见心房和左心室小梁区域的细胞有强烈标记，而右心室中表达ANF基因的细胞较少。心房和心室细胞中ANF基因表达的发育调控表明该肽激素在胚胎中起重要作用。ANF基因的局部表达为胚胎发育过程中识别心脏细胞亚群提供了一个标记。胚胎心室ANF mRNA转录本的鉴定可能与成人心室中ANF基因的病理性重新表达有关。
