Ladenson P W, Bloch K D, Seidman J G
Division of Endocrinology, Sinai Hospital, Baltimore, Maryland 21205.
Endocrinology. 1988 Jul;123(1):652-7. doi: 10.1210/endo-123-1-652.
The effect of thyroid hormone on atrial natriuretic factor (ANF) production was investigated in hypothyroid, euthyroid, and hyperthyroid rats by measuring levels of ANF mRNA and ANF in myocardium. ANF mRNA was quantitated by dot blot hybridization, and ANF by specific RIA. Relative ANF mRNA concentrations (ANF mRNA to 18S RNA) were determined for right atria, left atria, and ventricular apices. The total chamber content of ANF mRNA was estimated (concentration X total chamber RNA) and used as a measure of each tissue's synthetic capacity. For both atrial tissues, ANF mRNA contents were significantly higher in hyperthyroidism. In right atria, mean ANF mRNA contents in hypothyroidism and hyperthyroidism were 41% and 176%, respectively, of that in euthyroidism (P less than 0.05, by analysis of variance). Left atrial ANF mRNA contents in hypothyroidism and hyperthyroidism were 94% and 272%, respectively, of the euthyroid value (P less than 0.05). In contrast, atrial ANF mRNA concentrations did not differ significantly between thyroid states. In ventricle, ANF mRNA content and concentration were both correlated with serum T4 concentration. Ventricular ANF mRNA contents in hypothyroidism and hyperthyroidism were 31% and 178%, respectively, of that in euthyroidism (P less than 0.02). The concentration of ventricular ANF mRNA was also significantly increased in hyperthyroidism (P less than 0.05). Tissue content of ANF increased in the hyperthyroid right atria and decreased in the hyperthyroid left atria and ventricles. These observations suggest that increased ANF production by both atria and, to a lesser extent, by the ventricles contributes to the higher circulating ANF levels reported in hyperthyroidism. Furthermore, hyperthyroidism is associated with a specific increase in ventricular ANF mRNA expression as has been observed in other conditions causing ventricular hypertrophy.
通过测量心肌中心房利钠因子（ANF）mRNA和ANF的水平，研究甲状腺激素对甲状腺功能减退、甲状腺功能正常和甲状腺功能亢进大鼠心房利钠因子产生的影响。通过斑点杂交法定量ANF mRNA，通过特异性放射免疫分析法测定ANF。测定右心房、左心房和心室尖的相对ANF mRNA浓度（ANF mRNA与18S RNA之比）。估计ANF mRNA的总腔含量（浓度×总腔RNA），并将其用作每个组织合成能力的指标。对于两个心房组织，甲状腺功能亢进时ANF mRNA含量显著更高。在右心房，甲状腺功能减退和甲状腺功能亢进时的平均ANF mRNA含量分别为甲状腺功能正常时的41%和176%（方差分析，P<0.05）。甲状腺功能减退和甲状腺功能亢进时左心房ANF mRNA含量分别为甲状腺功能正常时的94%和272%（P<0.05）。相比之下，不同甲状腺状态下心房ANF mRNA浓度无显著差异。在心室中，ANF mRNA含量和浓度均与血清T4浓度相关。甲状腺功能减退和甲状腺功能亢进时心室ANF mRNA含量分别为甲状腺功能正常时的31%和178%（P<0.02）。甲状腺功能亢进时心室ANF mRNA浓度也显著增加（P<0.05）。甲状腺功能亢进的右心房中ANF的组织含量增加，而甲状腺功能亢进的左心房和心室中ANF的组织含量减少。这些观察结果表明，两个心房以及程度较轻的心室中ANF产生增加，导致了甲状腺功能亢进时报道的循环ANF水平升高。此外，甲状腺功能亢进与心室ANF mRNA表达的特异性增加有关，这在其他导致心室肥大的情况下也有观察到。
