Li Chao, Hu Xiaolu, Lu Jianyang, Mao Xiaoxia, Xiang Yang, Shu Yongqian, Li Genxi
State Key Laboratory of Pharmaceutical Biotechnology , Collaborative Innovation Center of Chemistry for Life Sciences , Department of Biochemistry , Nanjing University , Nanjing 210093 , P. R. China . Email:
Laboratory of Biosensing Technology , School of Life Sciences , Shanghai University , Shanghai , 200444 , China.
Chem Sci. 2017 Nov 28;9(4):979-984. doi: 10.1039/c7sc04663d. eCollection 2018 Jan 28.
Here we report a robust and sensitive DNA nanostructure-based electrochemical (E-nanoDNA) sensor that utilizes tetrahedral DNA nanostructures (TDNs) as an interfacial probe to detect biomolecules in a single-step procedure. In this study, we have firstly demonstrated that the use of TDNs can significantly suppress electrochemical background signals compared to traditional linear DNA probes upon introduction of base mismatches in the edges of TDNs. After further optimization of the two functional strands in the TDNs, quantitative, one-step detection of DNA can be achieved in the picomolar range in less than 10 min, and directly in complex media. Moreover, the baseline drift of this biosensor can be greatly decreased even after several hours in flowing whole blood , which suggests that the sensor holds potential to be employed in live animals. Furthermore, through replacing functional strands with aptamers or other DNA elements, this E-nanoDNA sensor can be easily used to probe various analytes, broadening the application range of the proposed sensor.
在此，我们报道了一种基于DNA纳米结构的强大且灵敏的电化学（E-nanoDNA）传感器，该传感器利用四面体DNA纳米结构（TDN）作为界面探针，通过单步程序检测生物分子。在本研究中，我们首先证明，与传统线性DNA探针相比，在TDN边缘引入碱基错配时，使用TDN可显著抑制电化学背景信号。在对TDN中的两条功能链进行进一步优化后，可在不到10分钟的时间内，在皮摩尔范围内直接在复杂介质中实现DNA的定量单步检测。此外，即使在流动全血中放置数小时后，该生物传感器的基线漂移也可大大降低，这表明该传感器具有在活体动物中应用的潜力。此外，通过用适体或其他DNA元件替换功能链，这种E-nanoDNA传感器可轻松用于探测各种分析物，拓宽了所提出传感器的应用范围。
