Hirao Shingo, Masumoto Hidetoshi, Itonaga Tatsuya, Minatoya Kenji
Department of Cardiovascular Surgery, Graduate School of Medicine, Kyoto University.
Department of Cardiovascular Surgery, Graduate School of Medicine, Kyoto University;
J Vis Exp. 2018 Mar 23(133):56986. doi: 10.3791/56986.
Cardiopulmonary bypass (CPB) is indispensable in cardiovascular surgery. Despite the dramatic refinement of CPB technique and devices, multi-organ complications related to prolonged CPB still compromise the outcome of cardiovascular surgeries, and may worsen postoperative morbidity and mortality. Animal models recapitulating the clinical usage of CPB enable the clarification of the pathophysiological processes that occur during CPB, and facilitate pre-clinical studies to develop strategies protecting against these complications. Rat CPB models are advantageous because of their greater cost-effectiveness, convenient experimental processes, abundant testing methods at the genetic or protein levels, and genetic consistency. They can be used for investigating the immune system activation and synthesis of proinflammatory cytokines, compliment activation, and production of oxygen free radicals. The rat models have been refined and have gradually taken the place of large-animal models. Here, we describe a simple CPB model without transfusion and/or inotropic agents in a rat. This recovery model allows the study of the long-term multiple organ sequelae of CPB.
体外循环（CPB）在心血管手术中不可或缺。尽管CPB技术和设备有了显著改进，但与长时间CPB相关的多器官并发症仍然会影响心血管手术的结果，并可能使术后发病率和死亡率恶化。模拟CPB临床应用的动物模型有助于阐明CPB期间发生的病理生理过程，并促进临床前研究以制定预防这些并发症的策略。大鼠CPB模型具有成本效益更高、实验过程方便、在基因或蛋白质水平上有丰富的检测方法以及基因一致性等优点。它们可用于研究免疫系统激活、促炎细胞因子的合成、补体激活以及氧自由基的产生。大鼠模型已经得到改进，并逐渐取代了大型动物模型。在此，我们描述一种在大鼠中不使用输血和/或正性肌力药物的简单CPB模型。这种恢复模型允许研究CPB的长期多器官后遗症。
