Lobel P, Dahms N M, Kornfeld S
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110.
J Biol Chem. 1988 Feb 15;263(5):2563-70.
We have isolated and sequenced cDNA clones encoding the entire sequence of the bovine cation-independent mannose 6-phosphate receptor. The deduced 2499-amino acid precursor has a calculated molecular mass of 275 kDa. Analysis of the sequence indicates that the protein has a 44-residue amino-terminal signal sequence, a 2269-residue extracytoplasmic region, a single 23-residue transmembrane region, and a 163-residue carboxyl-terminal cytoplasmic region. The extra-cytoplasmic region consists of 15 contiguous repeating domains, one of which contains a 43-residue insertion that is similar to the type II repeat of fibronectin. The 15 domains have an average size of 147 amino acids and a distinctive pattern of 8 cysteine residues. Alignment of the 15 domains and the extracytoplasmic domain of the cation-dependent mannose 6-phosphate receptor shows that all have sequence similarities and suggests that all are homologous.
我们已经分离并测序了编码牛源不依赖阳离子的甘露糖6-磷酸受体完整序列的cDNA克隆。推导的2499个氨基酸的前体计算分子量为275 kDa。序列分析表明，该蛋白质具有一个44个残基的氨基末端信号序列、一个2269个残基的胞外区域、一个23个残基的单跨膜区域和一个163个残基的羧基末端胞质区域。胞外区域由15个连续的重复结构域组成，其中一个结构域包含一个43个残基的插入序列，类似于纤连蛋白的II型重复序列。这15个结构域平均大小为147个氨基酸，具有独特的8个半胱氨酸残基模式。对依赖阳离子的甘露糖6-磷酸受体的15个结构域和胞外结构域进行比对，结果表明它们都具有序列相似性，提示它们都是同源的。
