长链非编码 RNA LET 对骨肉瘤细胞增殖和侵袭的影响。
Effect of lncRNA LET on proliferation and invasion of osteosarcoma cells.
机构信息
Department of Joint Orthopedics, Yantaishan Hospital, Yantai, Shandong, China.
出版信息
Eur Rev Med Pharmacol Sci. 2018 Mar;22(6):1609-1614. doi: 10.26355/eurrev_201803_14567.
OBJECTIVE
To investigate the expression of long non-coding RNA (LncRNA) LET in osteosarcoma and its effect on the proliferation, apoptosis, migration and invasion of osteosarcoma cells.
PATIENTS AND METHODS
The expression of lncRNA LET was detected in osteosarcoma tissues and cell lines (MG63 and hFOB1.19). MG63 cells stably overexpressing lncRNA LET were constructed by lentiviral. The effects of lncRNA LET overexpression on the proliferation, apoptosis, migration and invasion of osteosarcoma cells were detected by cell counting kit-8 (CCK-8), flow cytometry and transwell chamber assay.
RESULTS
The expression of lncRNA LET in osteosarcoma tissues and MG63 cells was significantly down-regulated. Overexpression of lncRNA LET significantly inhibited the proliferation, migration, invasion, and induced apoptosis of MG63 cells.
CONCLUSIONS
LncRNA LET was participated in the development of osteosarcoma, and may be used as a potential molecular target for the treatment of osteosarcoma.
目的
研究长链非编码 RNA（LncRNA）LET 在骨肉瘤中的表达及其对骨肉瘤细胞增殖、凋亡、迁移和侵袭的影响。
患者和方法
检测骨肉瘤组织和细胞系（MG63 和 hFOB1.19）中 lncRNA LET 的表达。通过慢病毒构建稳定过表达 lncRNA LET 的 MG63 细胞。通过细胞计数试剂盒-8（CCK-8）、流式细胞术和 Transwell 室测定检测 lncRNA LET 过表达对骨肉瘤细胞增殖、凋亡、迁移和侵袭的影响。
结果
lncRNA LET 在骨肉瘤组织和 MG63 细胞中的表达明显下调。过表达 lncRNA LET 显著抑制 MG63 细胞的增殖、迁移和侵袭，并诱导细胞凋亡。
结论
lncRNA LET 参与骨肉瘤的发生发展，可能成为骨肉瘤治疗的潜在分子靶点。
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