Department of Gastrointestinal Cancer Biology, National Clinical Research Center of Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China.
Eur Rev Med Pharmacol Sci. 2018 Mar;22(6):1665-1671. doi: 10.26355/eurrev_201803_14578.
The ubiquitin-conjugating enzyme E2C (UBE2C) has been known as a crucial factor upregulated in various tumors. The functions of UBE2C is mainly involved in the pathway protein ubiquitination. This study investigates the expression of UBE2C in gastric cancers and its correlation with overall survival rate.
Real-time PCR (RT-PCR) and Western blotting were performed to determine the expression of UBE2C in gastric cancer samples and adjacent normal tissues. Immunohistochemical staining was used to assess the expression of UBE2C in 216 paraffin-embedded gastric cancer tissues.
The mRNA and relevant protein levels of UBE2C in gastric cancer tissues are significantly greater than those in the adjacent normal tissues. Also, the expression of UBE2C is found to correlate with lymphatic metastasis, serosa invasion, TNM (Malignant Tumors) staging and Lauren's classification (p<0.05). The univariate analysis shows that the overexpression of UBE2C associates with poor prognosis (p=0.001). The multivariate analysis demonstrates that expression of UBE2C, lymphatic metastasis, and TNM staging are independent prognostic indicators.
This study shows that overexpression of UBE2C contributes to the development of gastric cancer, and UBE2C has the potential to be exploited as a therapeutic target.
泛素结合酶 E2C（UBE2C）已被证实为多种肿瘤中上调的关键因素。UBE2C 的功能主要涉及途径蛋白泛素化。本研究旨在探讨 UBE2C 在胃癌中的表达及其与总生存率的相关性。
采用实时 PCR（RT-PCR）和 Western 印迹法检测胃癌组织和相邻正常组织中 UBE2C 的表达。免疫组织化学染色法评估 216 例胃癌石蜡包埋组织中 UBE2C 的表达。
胃癌组织中 UBE2C 的 mRNA 和相关蛋白水平明显高于相邻正常组织。UBE2C 的表达与淋巴转移、浆膜侵犯、TNM（恶性肿瘤）分期和 Lauren 分类相关（p<0.05）。单因素分析显示，UBE2C 过表达与预后不良相关（p=0.001）。多因素分析表明，UBE2C 的表达、淋巴转移和 TNM 分期是独立的预后指标。
本研究表明 UBE2C 的过表达有助于胃癌的发展，UBE2C 有可能成为治疗靶点。
