Ghanbari Elham, Khazaei Mohammad Rasool, Ahangar Parinaz, Khazaei Mozafar
a Fertility and Infertility Research Center, Kermanshah University of Medical Sciences , Kermanshah , Iran.
b School of Pharmacy and Medical Sciences, University of South Australia , Adelaide , Australia.
J Diet Suppl. 2019;16(2):215-226. doi: 10.1080/19390211.2018.1448923. Epub 2018 Apr 9.
Crab shell (CS), which contains antioxidant compounds, has been used as a promising nutritional and medical compound in traditional medicine. Oxidative stress plays a critical role in the development of diabetes. This study aims to investigate the effects of CS extract (CSE) on sperm parameters, antioxidant status, and histopathology changes of testes in diabetic rats. In this experimental study, 40 Wistar male rats were investigated in five groups (n = 8/group): diabetic rats treated with different CS concentrations (i.e., 100, 200, and 400 mg/kg), diabetic group, and nondiabetic control. To induce diabetes, a single dose (60 mg/kg) of streptozotocin (STZ) was injected intraperitoneally; three days later, treatment with CSE was begun and conducted for 14 days. The fasting blood glucose, testes weight, and viability, number, and motility of sperm were assessed. In addition, the levels of ferric reducing antioxidant power (FRAP) and nitric oxide (NO) were estimated in the testes. Testes were examined using histological analysis. The results of this study revealed that sperm number, motility, and serum testosterone levels of CSE-treated diabetic rats increased significantly (p = .000) compared with the untreated diabetic group in a dose-depended manner while the number of immotile sperm decreased significantly (p = .017). CS also reduced the testicular level of nitric oxide and fasting blood glucose; however, it led to significant growth in the FRAP levels of testes (p = .002). Our results suggest that CSE improves sperm parameters and protects the testicular tissue against the oxidative stress damage induced by diabetes.
蟹壳（CS）含有抗氧化化合物，在传统医学中一直被用作一种有前景的营养和药用化合物。氧化应激在糖尿病的发展中起着关键作用。本研究旨在探讨CS提取物（CSE）对糖尿病大鼠精子参数、抗氧化状态和睾丸组织病理学变化的影响。在这项实验研究中，40只雄性Wistar大鼠被分为五组（每组n = 8）：分别用不同浓度CS（即100、200和400 mg/kg）治疗的糖尿病大鼠组、糖尿病组和非糖尿病对照组。通过腹腔注射单剂量（60 mg/kg）链脲佐菌素（STZ）诱导糖尿病；三天后开始用CSE治疗，并持续14天。评估空腹血糖、睾丸重量以及精子的活力、数量和运动能力。此外，还测定了睾丸中铁还原抗氧化能力（FRAP）和一氧化氮（NO）的水平。通过组织学分析对睾丸进行检查。本研究结果显示，与未治疗的糖尿病组相比，CSE治疗的糖尿病大鼠的精子数量、运动能力和血清睾酮水平显著增加（p = .000），且呈剂量依赖性，而不动精子的数量显著减少（p = .017）。CS还降低了睾丸中的一氧化氮水平和空腹血糖；然而，它导致睾丸中FRAP水平显著升高（p = .002）。我们的结果表明，CSE可改善精子参数，并保护睾丸组织免受糖尿病诱导的氧化应激损伤。
