1 Division of Cardiovascular Surgery, The 2nd Affiliated Hospital of Harbin Medical University , Harbin, China .
2 The Key Laboratory of Myocardial Ischemia, Ministry of Education, Harbin Medical University , Harbin, China .
Stem Cells Dev. 2018 May 1;27(9):612-623. doi: 10.1089/scd.2017.0271. Epub 2018 Apr 9.
Surgical ventricular reconstruction (SVR) can restore cardiac function for left ventricular aneurysm to some extent. However, the patches used in this treatment have some limitations such as stiffness and calcification. Engineering heart tissues (EHTs) have emerged as a promising biomaterial to repair damaged heart. Nevertheless, selecting optimal candidate cells for EHTs has been controversial. Aging is a major consideration for seed cells derived from elderly patients. Hence, this study was aimed to assess the proliferation of, antiapoptosis potential of, and expression of senescence-associated factors (eg, SA-β-Gal, cyclin-dependent kinase inhibitor 2A (P16), cyclin-dependent kinase inhibitor 1 (P21) in adipose-derived stem cells (ADSCs), and bone marrow stem cells (BMSCs) in vitro. In addition, cardiac function, cell survival, and angiogenesis of ADSCs and BMSCs after SVR were assessed in vivo. The in vitro results showed that old ADSCs (OAs) grew faster; expressed lower levels of SA-β-Gal, P16, and P21; and possessed more pronounced antiapoptosis activity than old BMSCs (OBs). The in vivo results demonstrated that 28 days after patch implantation, animals that received OAs patches showed better restoration of cardiac function than animals that received OBs patches. Meanwhile, old ADSCs possessed more potential regarding cell survival and angiogenesis. These results suggest that ADSCs may be superior to BMSCs with regard to autologous cell transplantation in elderly patients.
外科心室重构 (SVR) 可以在一定程度上恢复左心室动脉瘤的心脏功能。然而，该治疗中使用的补片存在一些局限性，如僵硬和钙化。工程心脏组织 (EHT) 已成为修复受损心脏的有前途的生物材料。然而，选择最佳的 EHT 候选细胞一直存在争议。衰老对于源自老年患者的种子细胞是一个主要考虑因素。因此，本研究旨在评估脂肪来源干细胞 (ADSCs) 和骨髓间充质干细胞 (BMSCs) 的体外增殖、抗凋亡潜力和衰老相关因子（如 SA-β-Gal、细胞周期蛋白依赖性激酶抑制剂 2A (P16)、细胞周期蛋白依赖性激酶抑制剂 1 (P21) 的表达。此外，还评估了 SVR 后 ADSCs 和 BMSCs 的心脏功能、细胞存活和血管生成。体外结果表明，老年 ADSCs (OAs) 生长更快；表达较低水平的 SA-β-Gal、P16 和 P21；并且具有更强的抗凋亡活性，比老年 BMSCs (OBs) 强。体内结果表明，在植入补片 28 天后，接受 OAs 补片的动物的心脏功能恢复优于接受 OBs 补片的动物。同时，老年 ADSCs 在细胞存活和血管生成方面具有更大的潜力。这些结果表明，与老年患者的自体细胞移植相比，ADSCs 可能优于 BMSCs。
