a Department of Laboratory Medicine , Daegu Fatima Hospital , Daegu , Republic of Korea.
b Department of Occupational Health , College of Bio-Medical Sciences, Daegu Catholic University , Daegu , Republic of Korea.
J Toxicol Environ Health A. 2018;81(12):502-511. doi: 10.1080/15287394.2018.1460785. Epub 2018 Apr 9.
Atopic dermatitis is a progressive inflammatory disease characterized by type 2 helper T cell (T2) reactivity. The aim of this study was to examine the therapeutic effects of Paeoniae radix alba root extract using a murine model of atopic dermatitis. Atopic dermatitis was induced in a murine model characterized by immune alterations skewed toward T2 reactivity and pathophysiological dermal alterations which resemble human atopic dermatitis. The root extract at 1% or 6% was applied to the mouse dorsal skin for 3 weeks following induction of atopic dermatitis. Splenocytes were stimulated with immobilized anti-CD3 for 48 h to measure cytokine production. Levels of serum IgE, IgG1, and IgG2a were quantitated. Epidermal thickness and numbers of skin mast cells were determined. Mice in which atopic dermatitis was induced displayed increased numbers of skin mast cells, increased frequency of scratching, elevated serum IgE levels, increased ratios of IgG1 to IgG2a, and ratios of IL-4 to IFN-γ. The frequency of scratching was significantly decreased following application of 1% or 6% extract for 1 week. The root extract also reversed T2 skewing, as serum IgE levels, ratio of serum IgG1 to IgG2a, and ratio of IL-4 to IFN-γ production by in vitro-stimulated splenocytes were all suppressed following application of 1% or 6% extract for 3 weeks. Taken together Paeoniae radix alba root extract is suggested to reverse the immunological alterations and skin manifestations symptoms found in atopic dermatitis.
特应性皮炎是一种进展性炎症性疾病，其特征是 2 型辅助 T 细胞（T2）反应。本研究旨在使用特应性皮炎小鼠模型来检验白芍根提取物的治疗效果。在免疫反应向 T2 反应倾斜且具有类似人类特应性皮炎的病理生理皮肤改变的特应性皮炎小鼠模型中诱导特应性皮炎。在诱导特应性皮炎后，将 1%或 6%的根提取物施用于小鼠背部皮肤 3 周。用固定化抗 CD3 刺激脾细胞 48 小时以测量细胞因子产生。定量血清 IgE、IgG1 和 IgG2a 水平。测定表皮厚度和皮肤肥大细胞数量。诱导特应性皮炎的小鼠显示皮肤肥大细胞数量增加，搔抓频率增加，血清 IgE 水平升高，IgG1 与 IgG2a 的比值以及 IL-4 与 IFN-γ 的比值增加。应用 1%或 6%提取物 1 周后，搔抓频率显著降低。根提取物还逆转了 T2 倾斜，因为体外刺激的脾细胞产生的血清 IgE 水平、血清 IgG1 与 IgG2a 的比值以及 IL-4 与 IFN-γ 的比值均在应用 1%或 6%提取物 3 周后受到抑制。综上所述，白芍根提取物可逆转特应性皮炎中发现的免疫改变和皮肤表现症状。
