Department and Program of Clinical Laboratory Medicine, Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan 430071, Hubei, China.
Department of Blood Transfusion, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China.
Cancer Biomark. 2018;22(1):135-142. doi: 10.3233/CBM-171109.
Circular RNAs (circRNA)are involved in the progression of cancers, and previous study showed that hsa_circ_0001649 expression is down-regulated in hepatocellular carcinoma (HCC).
To explore whether hsa_circ_0001649 is a prognostic biomarker for HCC and to investigate the biological functions of hsa_circ_0001649 in HCC.
Hsa_circ_0001649 expression was measured in 77 pairs of HCC and adjacent no-tumor tissues by quantitative Real-Time polymerase chain reaction. Kaplan-Meier curve and Cox regression were used to analyze its prognostic significance for HCC patients. In addition, the hsa_circ_0001649 was over-expressed using a circRNA-forming plasmid in HCC cells, and the biological function of hsa_circ_0001649 was investigated in vitro.
We verified that hsa_circ_0001649 was down-regulated in HCC tissues compared with adjacent non-tumor tissues. In addition, low hsa_circ_0001649 expression was associated with the poor overall survival of HCC patients, and Cox multivariate analysis showed that hsa_circ_0001649 is a novel independent prognostic factor for HCC patients. Furthermore, the in vitro experiments demonstrated that over-expressed hsa_circ_0001649 inhibits the proliferation, migration, and invasion and promotes the apoptosis of HCC cells.
Hsa_circ_0001649 could act as a novel prognostic biomarker for HCC patients. In addition, hsa_circ_ 0001649 might be a potential therapeutic target for HCC.
环状 RNA（circRNA）参与癌症的进展，先前的研究表明 hsa_circ_0001649 在肝细胞癌（HCC）中表达下调。
探讨 hsa_circ_0001649 是否为 HCC 的预后生物标志物，并研究 hsa_circ_0001649 在 HCC 中的生物学功能。
通过实时定量聚合酶链反应（qRT-PCR）测量 77 对 HCC 及相邻非肿瘤组织中的 hsa_circ_0001649 表达。Kaplan-Meier 曲线和 Cox 回归分析用于分析 hsa_circ_0001649 对 HCC 患者的预后意义。此外，使用 circRNA 形成质粒在 HCC 细胞中过表达 hsa_circ_0001649，并在体外研究 hsa_circ_0001649 的生物学功能。
我们验证了 hsa_circ_0001649 在 HCC 组织中较相邻非肿瘤组织表达下调。此外，低 hsa_circ_0001649 表达与 HCC 患者总体生存率差相关，Cox 多因素分析表明 hsa_circ_0001649 是 HCC 患者的新的独立预后因素。此外，体外实验表明，过表达 hsa_circ_0001649 可抑制 HCC 细胞的增殖、迁移和侵袭，促进其凋亡。
hsa_circ_0001649 可作为 HCC 患者的新型预后生物标志物。此外，hsa_circ_0001649 可能是 HCC 的潜在治疗靶点。
