Department of Nutrigenomics and Bromatology, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Katowice, Jednosci 8, 41-200 Sosnowiec, Poland.
Department of Nutrigenomics and Bromatology, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Katowice, Jednosci 8, 41-200 Sosnowiec, Poland.
Chem Biol Interact. 2018 May 1;287:13-19. doi: 10.1016/j.cbi.2018.04.004. Epub 2018 Apr 6.
Fluoride cytotoxicity has been associated with apoptosis, oxidative stress, general changes in DNA and RNA and protein biosynthesis, whereas the results of studies on the effect of SMF on antioxidant activity of cells are contradictory. Therefore, the aim of our study was to evaluate the simultaneous exposure of human cells to fluoride SMF that are generated by permanent magnets on the expression profile of the genes that are associated with the antioxidant defense system. Control fibroblasts and fibroblasts that had been treated with fluoride were subjected to the influence of SMF with a moderate induction. In order to achieve our aims, we applied modern molecular biology techniques such as the oligonucleotide microarray. Among the antioxidant defense genes, five (SOD1, PLK3, CLN8, XPA, HAO1), whose expression was significantly altered by the action of fluoride ions and the exposure to SMF were normalized their expression was identified. We showed that fluoride ions cause oxidative stress, whereas exposure to SMF with a moderate induction can suppress their effects by normalizing the expression of the genes that are altered by fluoride. Our research may explain the molecular mechanisms of the influence of fluoride and SMF that are generated by permanent magnets on cells.
氟化物的细胞毒性与细胞凋亡、氧化应激、DNA 和 RNA 以及蛋白质生物合成的普遍变化有关，而关于电磁场对细胞抗氧化活性影响的研究结果却相互矛盾。因此，我们的研究目的是评估人细胞同时暴露于由永磁体产生的氟化物电磁场对与抗氧化防御系统相关的基因表达谱的影响。对照成纤维细胞和经氟化物处理的成纤维细胞受到适度诱导的电磁场影响。为了实现我们的目标，我们应用了现代分子生物学技术，如寡核苷酸微阵列。在抗氧化防御基因中，有五个（SOD1、PLK3、CLN8、XPA、HAO1）的表达因氟离子的作用和电磁场的暴露而发生明显改变，我们确定了它们的表达被正常化。我们表明，氟离子会引起氧化应激，而适度诱导的电磁场暴露可以通过正常化氟化物改变的基因的表达来抑制其作用。我们的研究可以解释氟化物和由永磁体产生的电磁场对细胞影响的分子机制。
