Ledwon Joanna K, Turin Sergey Y, Gosain Arun K, Topczewska Jolanta M
Northwestern University Feinberg School of Medicine, Ann & Robert H. Lurie Children's Hospital of Chicago, Division of Plastic and Reconstructive Surgery, Stanley Manne Children's Research Institute, Chicago, IL, USA.
Northwestern University Feinberg School of Medicine, Ann & Robert H. Lurie Children's Hospital of Chicago, Division of Plastic and Reconstructive Surgery, Stanley Manne Children's Research Institute, Chicago, IL, USA.
Gene Expr Patterns. 2018 Sep;29:32-38. doi: 10.1016/j.gep.2018.04.002. Epub 2018 Apr 6.
Fibroblast growth factor (FGF) signaling is essential for many developmental processes and plays a pivotal role in skeletal homeostasis, regeneration and wound healing. FGF signals through one of five tyrosine kinase receptors: Fgfr1a, -1b, -2, -3, -4. To characterize the expression of zebrafish fgfr3 from the larval stage to adulthood, we used RNAscope in situ hybridization on paraffin sections of the zebrafish head. Our study revealed spatial and temporal distribution of fgfr3 transcript in chondrocytes of the head cartilages, osteoblasts involved in bone formation, ventricular zone of the brain, undifferentiated mesenchymal cells of the skin, and lens epithelium of the eye. In general, the expression pattern of zebrafish fgfr3 is similar to the expression observed in higher vertebrates.
成纤维细胞生长因子（FGF）信号传导对许多发育过程至关重要，在骨骼稳态、再生和伤口愈合中起关键作用。FGF通过五种酪氨酸激酶受体之一发出信号：Fgfr1a、-1b、-2、-3、-4。为了表征斑马鱼fgfr3从幼体阶段到成年期的表达情况，我们在斑马鱼头部的石蜡切片上使用了RNAscope原位杂交技术。我们的研究揭示了fgfr3转录本在头部软骨的软骨细胞、参与骨形成的成骨细胞、脑的脑室区、皮肤的未分化间充质细胞和眼的晶状体上皮中的时空分布。一般来说，斑马鱼fgfr3的表达模式与在高等脊椎动物中观察到的表达相似。
