School of Environmental Science and Engineering, Sun Yat-Sen University, Guangzhou 510275, China; Environmental Microbiome Research Center, Sun Yat-Sen University, Guangzhou 510275, China; Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Guangzhou 510275, China.
School of Environmental Science and Engineering, Sun Yat-Sen University, Guangzhou 510275, China; Environmental Microbiome Research Center, Sun Yat-Sen University, Guangzhou 510275, China.
Biotechnol Adv. 2018 Jul-Aug;36(4):1194-1206. doi: 10.1016/j.biotechadv.2018.03.018. Epub 2018 Apr 6.
In situ remediation employing organohalide-respiring bacteria represents a promising solution for cleanup of persistent organohalide pollutants. The organohalide-respiring bacteria conserve energy by utilizing H or organic compounds as electron donors and organohalides as electron acceptors. Reductive dehalogenase (RDase), a terminal reductase of the electron transport chain in organohalide-respiring bacteria, is the key enzyme that catalyzes halogen removal. Accumulating experimental evidence thus far suggests that there are distinct models for respiratory electron transfer in organohalide-respirers of different lineages, e.g., Dehalococcoides, Dehalobacter, Desulfitobacterium and Sulfurospirillum. In this review, to connect the knowledge in organohalide-respiratory electron transport chains to bioremediation applications, we first comprehensively review molecular components and their organization, together with energetics of the organohalide-respiratory electron transport chains, as well as recent elucidation of intramolecular electron shuttling and halogen elimination mechanisms of RDases. We then highlight the implications of organohalide-respiratory electron transport chains in stimulated bioremediation. In addition, major challenges and further developments toward understanding the organohalide-respiratory electron transport chains and their bioremediation applications are identified and discussed.
利用有机卤化物呼吸细菌进行原位修复代表了一种有前途的方法，可以清除持久性有机卤化物污染物。有机卤化物呼吸细菌通过利用 H 或有机化合物作为电子供体和有机卤化物作为电子受体来保存能量。还原脱卤酶 (RDase) 是有机卤化物呼吸细菌电子传递链的末端还原酶，是催化卤化物去除的关键酶。迄今为止，越来越多的实验证据表明，不同谱系的有机卤化物呼吸菌的呼吸电子传递存在明显的模型，例如 Dehalococcoides、Dehalobacter、Desulfitobacterium 和 Sulfurospirillum。在这篇综述中，为了将有机卤化物呼吸电子传递链的知识与生物修复应用联系起来，我们首先全面回顾了有机卤化物呼吸电子传递链的分子组成及其组织，以及能量学，以及 RDases 的分子内电子穿梭和卤化物消除机制的最新阐明。然后，我们强调了有机卤化物呼吸电子传递链在刺激生物修复中的意义。此外，还确定并讨论了理解有机卤化物呼吸电子传递链及其生物修复应用的主要挑战和进一步发展。
