Richie E R, Hilliard J K, Gilmore R, Gillespie D J
Department of Pediatrics, University of Texas Medical School, Houston.
J Reprod Immunol. 1987 Oct;12(2):137-48. doi: 10.1016/0165-0378(87)90041-6.
Purified lactoferrin (LF), isolated from human milk, was tested for its effect on human T-lymphocyte proliferative responses to phytohemagglutinin (PHA) and to alloantigens in mixed lymphocyte culture. LF inhibited proliferation in both assays in a dose-dependent manner. The suppressive effect was not due to LF mediated cytotoxicity since washing cells that had been pre-incubated with LF restored their proliferative activity. LF was most effective in suppressing the PHA response when added within 24 h of culture initiation. Iron saturated LF failed to inhibit PHA-induced proliferation, suggesting that the mechanisms of suppression involve the chelating property of LF. The suppressive effect of LF on T-lymphocyte proliferative response in vitro supports the notion that LF has significant immunoregulatory potential in vivo.
从人乳中分离出的纯化乳铁蛋白（LF），就其对人T淋巴细胞对植物血凝素（PHA）以及混合淋巴细胞培养中同种异体抗原的增殖反应的影响进行了测试。LF在两种检测中均以剂量依赖性方式抑制增殖。这种抑制作用并非由LF介导的细胞毒性所致，因为洗涤预先与LF孵育过的细胞可恢复其增殖活性。在培养开始后24小时内添加LF时，其对PHA反应的抑制最为有效。铁饱和的LF未能抑制PHA诱导的增殖，这表明抑制机制涉及LF的螯合特性。LF在体外对T淋巴细胞增殖反应的抑制作用支持了LF在体内具有显著免疫调节潜力的观点。
