Roh Ji-Yeon, Kim Pil-Gon, Kwon Jung-Hwan
Division of Environmental Science and Ecological Engineering, Korea University, Seoul, Korea.
Unit of Industrial Chemical & Biocides, Knoell Korea Ltd., Seoul, Korea.
Environ Health Toxicol. 2018 Feb 26;33(1):e2018006. doi: 10.5620/eht.e2018006. eCollection 2018.
Oxidative stress was evaluated for anthracene (Ant) and alkyl-Ants (9-methylanthracene [9-MA] and 9,10-dimethylanthracene [9,10-DMA]) in to compare changes in toxicity due to the degree of alkylation. Worms were exposed at 1) the same external exposure concentration and 2) the maximum water-soluble concentration. Formation of reactive oxygen species, superoxide dismutase activity, total glutathione concentration, and lipid peroxidation were determined under constant exposure conditions using passive dosing. The expression of oxidative stress-related genes ( and cytochrome 35A/C family genes) was also investigated to identify and compare changes in the genetic responses of exposed to Ant and alkyl-Ant. At the same external concentration, 9,10-DMA induced the greatest oxidative stress, as evidenced by all indicators, except for lipid peroxidation, followed by 9-MA and Ant. Interestingly, 9,10-DMA led to greater oxidative stress than 9-MA and Ant when worms were exposed to the maximum water-soluble concentration, although the maximum water-soluble concentration of 9,10-DMA is the lowest. Increased oxidative stress by alkyl-Ants would be attributed to higher lipid-water partition coefficient and the π electron density in aromatic rings by alkyl substitution, although this supposition requires further confirmation.
为了比较由于烷基化程度导致的毒性变化，对蒽（Ant）和烷基蒽（9-甲基蒽[9-MA]和9,10-二甲基蒽[9,10-DMA]）的氧化应激进行了评估。将蠕虫暴露于1）相同的外部暴露浓度和2）最大水溶性浓度下。在恒定暴露条件下使用被动给药法测定活性氧的形成、超氧化物歧化酶活性、总谷胱甘肽浓度和脂质过氧化。还研究了氧化应激相关基因（和细胞色素35A/C家族基因）的表达，以识别和比较暴露于Ant和烷基Ant的蠕虫的遗传反应变化。在相同的外部浓度下，9,10-DMA诱导的氧化应激最大，除脂质过氧化外，所有指标均证明了这一点，其次是9-MA和Ant。有趣的是，当蠕虫暴露于最大水溶性浓度时，9,10-DMA导致的氧化应激比9-MA和Ant更大，尽管9,10-DMA的最大水溶性浓度是最低的。烷基Ant导致的氧化应激增加可能归因于较高的脂水分配系数和烷基取代导致的芳香环中的π电子密度，尽管这一假设需要进一步证实。
