L.花水提取物对乙二醇诱导的大鼠肾结石的预防作用。

Preventive effects of the aqueous extract of L. flower on ethylene glycol-induced renal calculi in rats.

作者信息

Emamiyan Mahdieh Zaman, Vaezi Gholamhassan, Tehranipour Maryam, Shahrohkabadi Khdije, Shiravi Abdolhossein

机构信息

Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran.

Department of Biology, Faculty of Sciences, Sciences and Research Branch, Islamic Azad University, Tehran, Iran.

出版信息

Avicenna J Phytomed. 2018 Mar-Apr;8(2):170-178.

PMID:29632848

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5885331/

Abstract

OBJECTIVE

Urolithiasis remains a global problem. Despite the availability of numerous methods, no definite therapeutic agent has been yet introduced for the prevention or treatment of kidney stones. In this study, we evaluated the possible preventive effects of aqueous extract of L. (chicory) flowers on ethylene glycol-induced renal calculi in rats.

MATERIALS AND METHODS

A total of 24 Wistar rats were randomly divided into four groups and were treated for 30 days. Group A received drinking tap water, while groups B, C, and D were administered with 1% ethylene glycol for induction of calcium oxalate stone formation. Rats in groups C and D received intraperitoneal injections of the aqueous extract of chicory flowers (50 and 200 mg/kg, respectively) since the first day of the experiment. The urine volume, urine pH, and urinary levels of oxalate, citrate, calcium, uric acid, and creatinine as well as serum levels of calcium, uric acid, and creatinine were measured. After 30 days, the rats' kidneys were removed and prepared for histological evaluation of calcium oxalate deposits. One-way analysis of variance (ANOVA), followed by Tukey's test, was performed, using SPSS version 20.

RESULTS

The number of calcium oxalate crystals was significantly higher in group B (ethylene glycol-only treated animals), compared to group A (control), group C (50 mg/kg of aqueous extract), and group D (200 mg/kg of aqueous extract) (p<0.05). On day 30, the urine level of citrate, oxalate (p>0.05), and creatinine (p<0.05), as well as urine pH (p<0.05) decreased in groups C and D, compared to group B. Also, urine calcium level, urine uric acid (p>0.05), and urine volume (p<0.05) were higher in group D, compared to group B. In addition, the serum level of calcium, creatinine (p<0.05), and uric acid (p<0.001) decreased in groups C and D.

CONCLUSION

The aqueous extract of chicory flower (50 mg/kg) could reduce the number of calcium oxalate deposits in the urine and reduce the level of serum parameters.

摘要

目的

尿石症仍然是一个全球性问题。尽管有多种方法可用，但尚未有明确的治疗药物用于预防或治疗肾结石。在本研究中，我们评估了菊苣花水提取物对乙二醇诱导的大鼠肾结石的可能预防作用。

材料与方法

将24只Wistar大鼠随机分为四组，治疗30天。A组饮用自来水，而B、C、D组给予1%乙二醇以诱导草酸钙结石形成。自实验第一天起，C组和D组大鼠分别腹腔注射菊苣花水提取物（分别为50和200mg/kg）。测量尿量、尿pH值以及草酸、柠檬酸盐、钙、尿酸和肌酐的尿水平，以及钙、尿酸和肌酐的血清水平。30天后，取出大鼠肾脏，准备进行草酸钙沉积的组织学评估。使用SPSS 20版进行单因素方差分析（ANOVA），随后进行Tukey检验。

结果

与A组（对照组）、C组（50mg/kg水提取物）和D组（200mg/kg水提取物）相比，B组（仅用乙二醇处理的动物）中草酸钙晶体的数量显著更高（p<0.05）。在第30天，与B组相比，C组和D组的柠檬酸盐、草酸盐（p>0.05）和肌酐（p<0.05）尿水平以及尿pH值（p<0.05）降低。此外，与B组相比，D组的尿钙水平、尿尿酸（p>0.05）和尿量（p<0.05）更高。此外，C组和D组的血清钙、肌酐（p<0.05）和尿酸（p<0.001）水平降低。