Tsougeni Katerina, Ellinas Kosmas, Koukouvinos George, Petrou Panagiota S, Tserepi Angeliki, Kakabakos Sotirios E, Gogolides Evangelos
Institute of Nanoscience and Nanotechnology, NCSR Demokritos, Attiki, Greece.
Nanoplasmas PC, A Spin-Out Company of NCSR Demokritos, Lefkippos Technology Park, Attiki, Greece.
Methods Mol Biol. 2018;1771:27-40. doi: 10.1007/978-1-4939-7792-5_3.
Plasma micro-nanotexturing is a generic technology for topographical and chemical modification of surfaces and their implementation in microfluidics and microarrays. Nanotextured surfaces with desirable chemical functionality (and wetting behavior) have shown excellent biomolecule immobilization and cell adhesion. Specifically, nanotextured hydrophilic areas show (a) strong binding of biomolecules and (b) strong adhesion of cells, while nanotextured superhydrophobic areas show null adsorption of (a) proteins and (b) cells. Here we describe the protocols for (a) biomolecule adsorption control on nanotextured surfaces for microarray fabrication and (b) cell adhesion on such surfaces. 3D plasma nanotextured® substrates are commercialized through Nanoplasmas private company, a spin-off of the National Centre for Scientific Research Demokritos.
等离子体微纳纹理化是一种用于表面形貌和化学改性及其在微流体和微阵列中应用的通用技术。具有理想化学功能（和润湿性）的纳米纹理化表面已显示出优异的生物分子固定和细胞粘附性能。具体而言，纳米纹理化的亲水区域表现出：（a）生物分子的强结合，以及（b）细胞的强粘附；而纳米纹理化的超疏水区域则表现出：（a）蛋白质和（b）细胞的零吸附。在此，我们描述了用于（a）微阵列制造中纳米纹理化表面上生物分子吸附控制，以及（b）此类表面上细胞粘附的方案。3D等离子体纳米纹理化®基板通过国家科学研究德谟克利特中心的衍生公司Nanoplasmas私人公司实现商业化。
