Silva-Gutierrez N, Bahsas Zaky R, Bouchard M, Teran Angel G, Amoroso A, Peterson D L, Salmen S
Facultad de Medicina, Instituto de Inmunologia Clinica, Universidad de Los Andes, Mérida, Venezuela.
Department of Biochemistry, Virginia Commonwealth University, Richmond, VA, USA.
Parasite Immunol. 2018 Jun;40(6):e12532. doi: 10.1111/pim.12532. Epub 2018 Apr 30.
Toxoplasma gondii (T. gondii) is an obligate intracellular protozoan parasite that can infect almost all warm-blooded species and induce a chronic infection in human hosts. The aim of this work was to investigate Th1, Th2, Th17 and Treg polarization, induced by four important T. gondii antigens (SAG1, ROP1, GRA8 and MAG1) in acutely and chronically infected patients. For this purpose, SAG1, ROP1, GRA8 and MAG1 were expressed as recombinant proteins, purified, and used to evaluate the proinflammatory and regulatory immune response profiles in seropositive and seronegative individuals. Our results show that SAG1 and ROP1 elicited a proinflammatory profile (INF-γ, IL-12 and IL-17) in individuals in the acute phase, whereas MAG1 and GRA8 induced a regulatory pattern (Treg and TGF-β) in chronically infected patients. These results reveal fundamental differences in T-cell polarization induced by T. gondii antigens, which could have important implications in the immunopathogenesis of the disease and in future proposals of therapeutic strategies.
刚地弓形虫是一种专性细胞内原生动物寄生虫，可感染几乎所有温血动物，并在人类宿主中引发慢性感染。本研究旨在调查四种重要的刚地弓形虫抗原（SAG1、ROP1、GRA8和MAG1）在急性和慢性感染患者中诱导的Th1、Th2、Th17和Treg极化情况。为此，将SAG1、ROP1、GRA8和MAG1表达为重组蛋白，进行纯化，并用于评估血清阳性和血清阴性个体的促炎和调节性免疫反应谱。我们的结果表明，SAG1和ROP1在急性期个体中引发了促炎谱（INF-γ、IL-12和IL-17），而MAG1和GRA8在慢性感染患者中诱导了调节模式（Treg和TGF-β）。这些结果揭示了刚地弓形虫抗原诱导的T细胞极化的根本差异，这可能对该疾病的免疫发病机制以及未来治疗策略的提议具有重要意义。
