Regional Medical Research Centre (ICMR), Port Blair, Andaman and Nicobar Islands, 744101, India.
Department of Chemical Sciences, Ariel University, Ariel, 70400, Israel.
Proteins. 2018 Jul;86(7):712-722. doi: 10.1002/prot.25505. Epub 2018 Apr 19.
Proteomes of pathogenic Leptospira interrogans and L. borgpetersenii and the saprophytic L. biflexa were filtered through computational tools to identify Outer Membrane Proteins (OMPs) that satisfy the required biophysical parameters for their presence on the outer membrane. A total of 133, 130, and 144 OMPs were identified in L. interrogans, L. borgpetersenii, and L. biflexa, respectively, which forms approximately 4% of proteomes. A holistic analysis of transporting and pathogenic characteristics of OMPs together with Clusters of Orthologous Groups (COGs) among the OMPs and their distribution across 3 species was made and put forward a set of 21 candidate OMPs specific to pathogenic leptospires. It is also found that proteins homologous to the candidate OMPs were also present in other pathogenic species of leptospires. Six OMPs from L. interrogans and 2 from L. borgpetersenii observed to have similar COGs while those were not found in any intermediate or saprophytic forms. These OMPs appears to have role in infection and pathogenesis and useful for anti-leptospiral strategies.
通过计算工具筛选致病性钩端螺旋体（Leptospira interrogans）和伯氏钩端螺旋体（L. borgpetersenii）以及腐生性双曲钩端螺旋体（L. biflexa）的蛋白质组，以鉴定满足其在外膜上存在的所需生物物理参数的外膜蛋白（OMPs）。分别在 L. interrogans、L. borgpetersenii 和 L. biflexa 中鉴定到了 133、130 和 144 个 OMPs，分别约占蛋白质组的 4%。对 OMPs 的转运和致病特征以及 OMPs 之间的同源基因簇（COGs）进行整体分析，并将其分布在 3 个种属中进行分析，提出了一组 21 种与致病性钩端螺旋体特异性相关的候选 OMPs。还发现候选 OMPs 的同源蛋白也存在于其他致病性钩端螺旋体物种中。从 L. interrogans 中观察到 6 种 OMPs 和 L. borgpetersenii 中的 2 种 OMPs 具有相似的 COGs，而在任何中间或腐生形式中都未发现这些 COGs。这些 OMPs 似乎在感染和发病机制中发挥作用，对抗钩端螺旋体策略有用。
