Zhou Zuoyong, Wu Yutong, Chen Yiwang, Wang Zhiying, Hu Shijun, Zhou Rongqiong, Dong Chunxia, Lin Hongquan, Nie Kui
College of Animal Science, Rongchang Campus of Southwest University, No. 160 Xueyuan Road, Rongchang District, Chongqing 402460, PR China - Veterinary Science Engineering Research Center of Chongqing, No. 160 Xueyuan Road, Rongchang District, Chongqing 402460, PR China.
Guizhou Institute of Animal Husbandry and Veterinary Medicine, No. 2 Laolipo, Naming District, Guizhou 550005, PR China.
Parasite. 2018;25:20. doi: 10.1051/parasite/2018024. Epub 2018 Apr 10.
Toxoplasmosis and anaplasmosis are severe zoonotic diseases, the former caused by Toxoplasma gondii and the latter by Anaplasma spp. In the present study, 332 goat blood samples were randomly collected from Chongqing Municipality, China to screen for T. gondii and Anaplasma spp. We used a polymerase chain reaction (PCR) to detect DNA, and enzyme-linked immunosorbent assay (ELISA) to test for T. gondii antibodies. The prevalence of T. gondii and Anaplasma spp. was 38% and 35% respectively by PCR, and 42% for T. gondii antibodies by ELISA. The co-infection rate by T. gondii and Anaplasma was 13%, where the two predominant pathogens co-infecting were Anaplasma phagocytophilum + A. bovis (10%), followed by T. gondii + A. phagocytophilum (9.64%). While co-infection by three pathogens varied ranging from 1.81% to 5.72%, less than 1% of goats were found to be positive for four pathogens. This is the first investigation of T. gondii and Anaplasma spp. infection in goats from Chongqing.
弓形虫病和无形体病是严重的人畜共患病，前者由刚地弓形虫引起，后者由无形体属细菌引起。在本研究中，从中国重庆市随机采集了332份山羊血样，以筛查刚地弓形虫和无形体属细菌。我们使用聚合酶链反应（PCR）检测DNA，并使用酶联免疫吸附测定（ELISA）检测刚地弓形虫抗体。通过PCR检测，刚地弓形虫和无形体属细菌的感染率分别为38%和35%，通过ELISA检测，刚地弓形虫抗体阳性率为42%。刚地弓形虫和无形体的共感染率为13%，其中两种主要的共感染病原体是嗜吞噬细胞无形体+牛无形体（10%），其次是刚地弓形虫+嗜吞噬细胞无形体（9.64%）。三种病原体的共感染率在1.81%至5.72%之间变化，发现不到1%的山羊四种病原体检测均为阳性。这是对重庆山羊刚地弓形虫和无形体属细菌感染的首次调查。
