泰国北部山羊血样中[具体物质]遗传物质的分子检测。（你原文中“of ”后面缺少具体内容）

Molecular Detection of Genetic Material of in Goat Blood Samples from Northern Thailand.

作者信息

Koonyosying Pongpisid, Muenthaisong Anucha, Sangkakam Kanokwan, Nontasaya Kanpitcha, Rittipornlertrak Amarin, Nambooppha Boondarika, Apinda Nisachon, Maneekesorn Supawadee, Sthitmatee Nattawooti

机构信息

Laboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand.

Office of Research Administration, Chiang Mai University, Chiang Mai 50200, Thailand.

出版信息

Vet Sci. 2025 Jun 5;12(6):555. doi: 10.3390/vetsci12060555.

DOI:10.3390/vetsci12060555

PMID:40559792

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12197340/

Abstract

Toxoplasmosis is a significant parasitic zoonosis caused by . Among livestock animals, small ruminants, especially sheep and goats, are the most infected. This infection is a leading cause of abortion, resulting in considerable economic losses for goat breeders. The present study aimed to assess the prevalence of infection in goats in northern Thailand, with an emphasis on its potential zoonotic transmission to humans. Polymerase chain reaction (PCR) targeting the dihydrofolate reductase-thymidylate synthase () gene was employed for molecular detection. This represents the first report of molecular detection in blood samples from small ruminants in Thailand by PCR. A total of 176 meat goat blood samples were analyzed, yielding an 8.52% (15/176) positivity rate for DNA. The selected DNA sequences from the positive DNA displayed a high degree of nucleotide sequence homology with the reference sequence. Phylogenetic analysis revealed a single clade alongside other strains, showing no differentiation based on genotype. This study contributes to the understanding of epidemiology and provides a foundation for future strategies to control and manage transmission in livestock populations.

摘要

弓形虫病是一种由……引起的重要寄生虫人畜共患病。在家畜中，小型反刍动物，尤其是绵羊和山羊，感染最为严重。这种感染是导致流产的主要原因，给山羊养殖户造成了相当大的经济损失。本研究旨在评估泰国北部山羊的……感染率，重点关注其向人类潜在的人畜共患病传播。针对……二氢叶酸还原酶-胸苷酸合成酶（……）基因的聚合酶链反应（PCR）用于分子检测。这是泰国首次通过PCR对小型反刍动物血液样本进行……分子检测的报告。共分析了176份肉用山羊血液样本，……DNA的阳性率为8.52%（15/176）。从阳性……DNA中选择的DNA序列与参考……序列显示出高度的核苷酸序列同源性。系统发育分析显示与其他……菌株在一个单一支系中，基于基因型没有分化。本研究有助于了解……流行病学，并为未来控制和管理家畜群体中……传播的策略提供基础。