Zhang Yan, Lv Yali, Zhang Feifei, Zhang Wenjing, Wang Jinhong, Cui Yanyan, Wang Rongjun, Jian Fuchun, Zhang Longxian, Ning Changshen
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
J Vet Sci. 2016 Dec 30;17(4):523-529. doi: 10.4142/jvs.2016.17.4.523.
Members of the genus are important emerging tick-borne pathogens in both humans and animals in tropical and subtropical areas. Here, we investigated the presence of spp. in 621 sheep and 710 goats from six provinces of China. Polymerase chain reaction (PCR) and DNA sequencing were conducted to determine the prevalence of and targeting the 16S ribosomal RNA or the major surface protein 4 gene. PCR revealed in 39.0% (240/621) of sheep and 45.5% (323/710) of goats. The most frequently detected species was (88/621, 14.2% for sheep; 129/710, 18.2% for goats), followed by (60/621, 9.7% for sheep; 74/710, 10.4% for goats) and (33/621, 5.3% for sheep; 15/710, 2.1% for goats). Additionally, eight sheep and 20 goats were found to be infected with three pathogens simultaneously. DNA sequencing confirmed the presence of these three species in the investigated areas, and phylogenetic analysis indicated that there was geographic segregation to a certain extent, as well as a relationship between the host and cluster of The results of the present study provide valuable data that helps understand the epidemiology of anaplasmosis in ruminants from China.
该属成员是热带和亚热带地区人和动物中重要的新兴蜱传病原体。在此，我们调查了中国六个省份621只绵羊和710只山羊中该属物种的存在情况。进行了聚合酶链反应（PCR）和DNA测序，以确定针对16S核糖体RNA或主要表面蛋白4基因的该属物种的流行情况。PCR检测发现，39.0%（240/621）的绵羊和45.5%（323/710）的山羊感染了该属物种。最常检测到的物种是[具体物种名称1]（绵羊中88/621，占14.2%；山羊中129/710，占18.2%），其次是[具体物种名称2]（绵羊中60/621，占9.7%；山羊中74/710，占10.4%）和[具体物种名称3]（绵羊中33/621，占5.3%；山羊中15/710，占2.1%）。此外，发现8只绵羊和20只山羊同时感染了三种病原体。DNA测序证实了所调查地区存在这三种该属物种，系统发育分析表明在一定程度上存在地理隔离，以及该属物种与宿主之间的关系。本研究结果提供了有价值的数据，有助于了解中国反刍动物无形体病的流行病学情况。
