a Department of Anatomy and Physiology, Shandong College of Traditional Chinese Medicine , Yantai , China.
Arch Physiol Biochem. 2019 Oct;125(4):321-331. doi: 10.1080/13813455.2018.1459728. Epub 2018 Apr 10.
Renal ischaemia reperfusion (I/R) is a common clinical condition with a high morbidity and mortality rate. To date, I/R-induced renal injury remains an ineffective treatment. We hypothesis that angiogenesis and lymphangiogenesis markers, prospero homeobox-1 (PROX-1) and lymphatic endothelial hyaluronan receptor-1 (LYVE-1), are critical during I/R. Kunming mice were subjected to I/R and observed for the following eight consecutive days. Pathology analysis and protein distribution were detected by H&E staining, immunohistochemistry and immunofluorescence confocal analysis. After I/R treatment, renal pathology was changed. HIF-1α was induced in the early stage and colocalisation with PROX-1 mainly in the renal tubular region, whereas PROX-1 and LYVE-1 were colocalised in the glomerulus of the endothelial region. In this study, we revealed HIF-1α/PROX-1/LVYE-1 axis dynamic changes in different regions after I/R and demonstrated for the first time it activates during I/R repair.
肾缺血再灌注（I/R）是一种常见的临床病症，具有较高的发病率和死亡率。迄今为止，I/R 引起的肾损伤仍然没有有效的治疗方法。我们假设血管生成和淋巴管生成标志物，prospero 同源盒蛋白 1（PROX-1）和淋巴管内皮透明质酸受体 1（LYVE-1），在 I/R 过程中至关重要。昆明小鼠接受 I/R 处理，并连续观察 8 天。通过 H&E 染色、免疫组织化学和免疫荧光共聚焦分析检测病理分析和蛋白分布。在 I/R 治疗后，肾脏病理发生改变。在早期诱导 HIF-1α，主要在肾小管区域与 PROX-1 共定位，而 PROX-1 和 LYVE-1 则在血管内皮区域的肾小球中共定位。在这项研究中，我们揭示了 I/R 后不同区域中 HIF-1α/PROX-1/LYVE-1 轴的动态变化，并首次证明它在 I/R 修复过程中被激活。
