Ezquer-Garin Carlos, Ferriols-Lisart Rafael, Alós-Almiñana Manuel, Aguilar-Aguilar Gerardo, Belda-Nacher Javier F, Carbonell Jose-Antonio
Institute for Health Research (INCLIVA), University Clinical Hospital of Valencia, Valencia, Spain.
Department of Pharmacy, University Clinical Hospital of Valencia, Valencia, Spain.
Bioanalysis. 2018 Apr 1;10(7):461-473. doi: 10.4155/bio-2017-0257. Epub 2018 Apr 10.
A simple, rapid, economical and sensitive HPLC-UV method was developed for the simultaneous quantification of ceftolozane and tazobactam in plasma samples.
After deproteinization followed by a liquid-liquid back-extraction, the compounds were separated on a C18 column (150 mm × 4.6 mm, 5 μm) with UV-visible detection at 220 nm. The mobile phase consisted of acetonitrile and potassium dihydrogenphosphate buffer at pH 3.0 (8:92, v/v), delivered isocratically at a flow rate of 1.0 ml/min and at a column oven temperature of 30°C. Cefepime was used as an internal standard.
Linearity was achieved in the concentration range of 0.50-100.00 μg/ml for ceftolozane and 0.25-50.00 μg/ml for tazobactam. The intra- and interday precision showed good reproducibility with coefficients of variation of less than 9.26% for ceftolozane and 9.62% for tazobactam.
The sample preparation procedure avoids expensive or time-consuming steps used by other previously published methods. The methodology was validated according to standard guidelines and was used for quantification of ceftolozane and tazobactam in plasma samples from critically ill patients.
建立一种简单、快速、经济且灵敏的高效液相色谱-紫外检测法，用于同时定量测定血浆样本中的头孢洛扎和他唑巴坦。
经去蛋白处理后进行液-液反萃取，化合物在C18柱（150 mm×4.6 mm，5μm）上分离，于220 nm处进行紫外可见检测。流动相由乙腈和pH 3.0的磷酸二氢钾缓冲液（8:92，v/v）组成，等度洗脱，流速为1.0 ml/min，柱温箱温度为30℃。头孢吡肟用作内标。
头孢洛扎在0.50 - 100.00μg/ml浓度范围内、他唑巴坦在0.25 - 50.00μg/ml浓度范围内呈线性。日内和日间精密度显示出良好的重现性，头孢洛扎的变异系数小于9.26%，他唑巴坦的变异系数小于9.62%。
样品制备过程避免了其他先前发表方法中使用的昂贵或耗时步骤。该方法按照标准指南进行了验证，并用于定量测定重症患者血浆样本中的头孢洛扎和他唑巴坦。
