Sutherland Christina A, Nicolau David P
Center for Anti-Infective Research and Development, Hartford Hospital, 80 Seymour Street, Hartford, CT 06102, USA.
Center for Anti-Infective Research and Development, Hartford Hospital, 80 Seymour Street, Hartford, CT 06102, USA Division of Infectious Diseases, Hartford Hospital, Hartford, CT, USA
J Chromatogr Sci. 2016 Jul;54(6):1037-40. doi: 10.1093/chromsci/bmw047. Epub 2016 Apr 4.
Herein, we report the development and validation of an HPLC method to analyze ceftolozane and tazobactam simultaneously in human plasma, human serum, swine serum and saline matrixes. A reversed-phase column was used with a UV detector set at 260 nm and switched to 218 nm. The mobile phase consisted of methanol and sodium phosphate buffer at a flow rate of 1.1 mL/min. Cefepime was used as the internal standard. The standard curves were linear over a range of 0.4-50 μg/mL. This methodology represents a simple, reproducible approach to the determination of drug concentrations with sufficient accuracy and precision for pharmacokinetic studies undertaken with this recently FDA-approved antimicrobial therapy.
在此，我们报告了一种高效液相色谱法的开发与验证，该方法可同时分析人血浆、人血清、猪血清和生理盐水基质中的头孢洛扎和他唑巴坦。使用反相柱，紫外检测器设置在260 nm，并切换至218 nm。流动相由甲醇和磷酸钠缓冲液组成，流速为1.1 mL/min。头孢吡肟用作内标。标准曲线在0.4 - 50 μg/mL范围内呈线性。该方法是一种简单、可重复的测定药物浓度的方法，对于采用这种最近获得FDA批准的抗菌治疗进行的药代动力学研究具有足够的准确性和精密度。
