Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan.
Analyst. 2018 Apr 30;143(9):2102-2108. doi: 10.1039/c8an00332g.
In this work, an application of a design of experiments approach for the optimization of an isoniazid assay on a single-area inkjet-printed paper-based analytical device (PAD) is described. For this purpose, a central composite design was used for evaluation of the effect of device geometry and amount of assay reagents on the efficiency of the proposed device. The factors of interest were printed length, width, and sampling volume as factors related to device geometry, and amounts of the assay reagents polyvinyl alcohol (PVA), NH4OH, and AgNO3. Deposition of the assay reagents was performed by a thermal inkjet printer. The colorimetric assay mechanism of this device is based on the chemical interaction of isoniazid, ammonium hydroxide, and PVA with silver ions to induce the formation of yellow silver nanoparticles (AgNPs). The in situ-formed AgNPs can be easily detected by the naked eye or with a simple flat-bed scanner. Under optimal conditions, the calibration curve was linear in the isoniazid concentration range 0.03-10 mmol L-1 with a relative standard deviation of 3.4% (n = 5 for determination of 1.0 mmol L-1). Finally, the application of the proposed device for isoniazid determination in pharmaceutical preparations produced satisfactory results.
在这项工作中，描述了一种实验设计方法在单区域喷墨印刷纸基分析器件（PAD）上优化异烟肼测定的应用。为此，使用中心复合设计来评估器件几何形状和测定试剂用量对所提出的器件效率的影响。感兴趣的因素是与器件几何形状有关的打印长度、宽度和采样体积，以及测定试剂聚乙烯醇（PVA）、氨和 AgNO3 的量。测定试剂的沉积通过热喷墨打印机进行。该器件的比色测定机制基于异烟肼、氨和 PVA 与银离子的化学相互作用，诱导形成黄色银纳米颗粒（AgNPs）。原位形成的 AgNPs 可以用肉眼或简单的平板扫描仪轻松检测到。在最佳条件下，校准曲线在 0.03-10 mmol L-1 的异烟肼浓度范围内呈线性，相对标准偏差为 3.4%（n = 5 用于测定 1.0 mmol L-1）。最后，所提出的器件在药物制剂中异烟肼测定的应用得到了令人满意的结果。
