Zeric Stosic Marina Z, Jaksic Sandra M, Stojanov Igor M, Apic Jelena B, Ratajac Radomir D
Acta Pol Pharm. 2016 Nov;73(6):1461-1466.
High-performance liquid chromatography (HPLC) method with diode array detection (DAD) were optimized and validated for separation and determination of tetramethrin in an antiparasitic human shampoo. In order to optimize separation conditions, two different columns, different column oven temperatures, as well as mobile phase composition and ratio, were tested. Best separation was achieved on the Supelcosil TM LC-18- DB column (4.6 x 250 mm), particle size 5 jim, with mobile phase methanol : water (78 : 22, v/v) at a flow rate of 0.8 mL/min and at temperature of 30⁰C. The detection wavelength of the detector was set at 220 nm. Under the optimum chromatographic conditions, standard calibration curve was measured with good linearity [r2 = 0.9997]. Accuracy of the method defined as a mean recovery of tetramethrin from shampoo matrix was 100.09%. The advantages of this method are that it can easily be used for the routine analysis of drug tetramethrin in pharmaceutical formulas and in all pharmaceutical researches involving tetramethrin.
采用二极管阵列检测（DAD）的高效液相色谱（HPLC）方法被优化并验证，用于分离和测定一种抗寄生虫的人体洗发水中的胺菊酯。为了优化分离条件，测试了两种不同的色谱柱、不同的柱温箱温度以及流动相的组成和比例。在Supelcosil TM LC - 18 - DB柱（4.6×250 mm，粒径5μm）上，以甲醇:水（78:22，v/v）为流动相，流速为0.8 mL/min，温度为30℃时实现了最佳分离。检测器的检测波长设定为220 nm。在最佳色谱条件下，测得标准校准曲线具有良好的线性[r2 = 0.9997]。该方法的准确度以胺菊酯从洗发水基质中的平均回收率表示为100.09%。该方法的优点是可轻松用于药物配方中胺菊酯的常规分析以及所有涉及胺菊酯的药物研究。
