Ma Yingtao, Zhao Xuejiao, Ren Hongwei, Wu Hongxia, Guo Mingxin, Zhang Yanzhao, He Zhaojun, Han Jianming, Tong Ruijian
Life Science College , Luoyang Normal University , Luoyang , Henan 471934 , China.
Institute of Fruit Science , Luoyang Academy of Agriculture and Forestry , Luoyang , Henan 471000 , China.
J Agric Food Chem. 2018 Apr 25;66(16):4051-4061. doi: 10.1021/acs.jafc.8b00207. Epub 2018 Apr 13.
Freshly consumed peaches ( Prunus persica L. Batsch) can cause allergic reactions in the worldwide population because of the presence of four classes of allergens (Pru p 1, Pru p 2, Pru p 3, and Pru p 4). Fruit bagging has been widely practiced in peach cultivation to improve fruit quality; however, its effect on the expression of peach allergen-encoding genes remains unknown. In this study, the influence of fruit bagging with opaque paper bags on the major peach allergen-encoding genes, including Pru p 1.01, Pru p 1.06B, Pru p 2.01B, Pru p 2.02, Pru p 3.01, Pru p 4.01, and Pru p 4.02, were measured by means of real-time PCR. A significant reduction in transcript accumulation was observed for all of the selected genes in the epicarps of the bagged peach fruits, whereas slight increases were observed in the mesocarps for these genes, with the two exceptions of Pru p 2.02 and Pru p 3.01. For most of these genes, much higher transcripts were determined in the epicarps than in the mesocarps. Taken together, a significant reduction in the transcription rate of the allergen-encoding genes in the whole peach fruit was achieved by shading with opaque paper bags. According to these data, modifications in growing practices of peach may help to obtain fruits with lower levels of allergens and thus contribute to reducing potential allergenic risks in consumers.
新鲜食用的桃子（Prunus persica L. Batsch）由于存在四类过敏原（Pru p 1、Pru p 2、Pru p 3和Pru p 4），可在全球人群中引起过敏反应。果实套袋已在桃树栽培中广泛应用以提高果实品质；然而，其对桃子过敏原编码基因表达的影响尚不清楚。在本研究中，通过实时PCR测定了用不透明纸袋套袋对主要桃子过敏原编码基因的影响，这些基因包括Pru p 1.01、Pru p 1.06B、Pru p 2.01B、Pru p 2.02、Pru p 3.01、Pru p 4.01和Pru p 4.02。在套袋桃子果实的外果皮中，所有选定基因的转录积累均显著降低，而在中果皮中，除Pru p 2.02和Pru p 3.01外，这些基因的转录略有增加。对于这些基因中的大多数，在外果皮中测定的转录本比在中果皮中高得多。总体而言，用不透明纸袋遮光可使整个桃子果实中过敏原编码基因的转录率显著降低。根据这些数据，改变桃子的种植方式可能有助于获得过敏原含量较低的果实，从而有助于降低消费者潜在的过敏风险。
