Department of Otolaryngology, First Affiliated Hospital of Shantou University Medical College , Shantou, Guangdong, China .
Cancer Biother Radiopharm. 2018 Mar;33(2):60-64. doi: 10.1089/cbr.2017.2254.
Nasopharyngeal carcinoma (NPC) shows the leading morbidity in otorhinolaryngological malignant tumor. It is a common malignancy in China with obvious reginal distribution. NPC is a polygenic disease that is affected by numerous factors. Protein tyrosine phosphatase nonreceptor type 12 (PTPN12) regulates multiple tumor proliferation and development, including breast cancer and colon cancer. However, the role of PTPN12 in NPC occurrence and development has not been elucidated.
NPC cell line CNE2 was cultured in vitro and divided into three groups, including control, empty plasmid, and PTPN12 groups. PTPN12 mRNA and protein expressions were tested by real-time polymerase chain reaction and Western blot. CNE2 cell proliferation was detected by MTT assay. Cell migration was determined by wound healing assay. Cell apoptosis was evaluated by caspase 3 activity detection. Epidermal growth factor receptor (EGFR) expression was assessed by Western blot.
PTPN12 plasmid transfection increased PTPN12 mRNA and protein expressions, suppressed cell proliferation and migration, reduced EGFR level, and enhanced caspase 3 activity compared with control and empty plasmid groups (p < 0.05).
PTPN12 regulates NPC proliferation and migration through negative regulating EGFR. It could be treated as a molecular target for NPC diagnosis and prognosis analysis.
鼻咽癌（NPC）在耳鼻喉恶性肿瘤中发病率居首位。它是中国常见的恶性肿瘤，具有明显的区域性分布。NPC 是一种多基因疾病，受多种因素影响。蛋白酪氨酸磷酸酶非受体型 12（PTPN12）调节多种肿瘤的增殖和发展，包括乳腺癌和结肠癌。然而，PTPN12 在 NPC 发生和发展中的作用尚未阐明。
体外培养 NPC 细胞系 CNE2，并将其分为三组，分别为对照组、空载质粒组和 PTPN12 组。采用实时聚合酶链反应和 Western blot 检测 PTPN12 mRNA 和蛋白表达。MTT 法检测 CNE2 细胞增殖。划痕愈合试验检测细胞迁移。Caspase 3 活性检测评估细胞凋亡。Western blot 检测表皮生长因子受体（EGFR）的表达。
与对照组和空载质粒组相比，PTPN12 质粒转染增加了 PTPN12 mRNA 和蛋白的表达，抑制了细胞增殖和迁移，降低了 EGFR 水平，并增强了 caspase 3 活性（p<0.05）。
PTPN12 通过负调控 EGFR 调节 NPC 的增殖和迁移。它可以作为 NPC 诊断和预后分析的分子靶点。
