Sámano Reyna, Huesca-Gómez Claudia, López-Marure Rebeca, Hernández-Cabrera Ana-Karen, Rodríguez-Ventura Ana, Tolentino Maricruz, Morales Rosa María, Gamboa Ricardo
Department of Nutrition and Bioprogramming, Instituto Nacional de Perinatología, Mexico City, Mexico.
Department of Physiology, Instituto Nacional de Cardiología "Ignacio Chávez" Juan Badiano No 1, Col. Sección XVI, Mexico City, Mexico.
J Pediatr Endocrinol Metab. 2018 Apr 25;31(5):561-568. doi: 10.1515/jpem-2017-0262.
It has been reported that the uncoupling proteins (UCPs) can contribute to energy metabolism, and are thus involved in the pathogenesis of obesity. The objective of the study was to analyze the association between UCP polymorphisms, clinical parameters and leptin and adiponectin plasma levels in an adolescent population with overweight and obesity.
We analyzed the UCP1 -3826 C/T, UCP2-866 G/A, Ala55Val and UCP3 -55 C/T polymorphisms and the levels of adipokines in adolescents with normal weight and with overweight or obesity. The study included 270 students aged between 12 and 18 years categorized according to the percentiles from Mexico City. Adipokines levels were measured by immunoassay methods and the UCP polymorphisms were determined using Taqman real-time polymerase chain reaction (RT-PCR).
No significant differences were found in the UCP polymorphisms in seven inheritance models studied. Most of the significant differences in the clinical parameters were found under a recessive model, the UCP2 -866 polymorphism was associated with diastolic blood pressure (p=0.008), triglycerides (p=0.045), low-density lipoprotein-cholesterol (LDL-C) (p=0.003), high-density lipoprotein-cholesterol (HDL-C) (p=0.050) and plasma levels of leptin (p<0.001). Also, the obese group was found to have higher leptin levels and lower adiponectin levels in GA+AA vs. GG (recessive model).
This study demonstrated a direct relationship between the clinical characteristics and UCP2-866 in a recessive model, associated with high levels of leptin and decreased levels of adiponectin in an obese or overweight Mexican adolescent population.
据报道，解偶联蛋白（UCPs）可促进能量代谢，因此参与肥胖症的发病机制。本研究的目的是分析超重和肥胖青少年人群中UCP基因多态性、临床参数与瘦素和脂联素血浆水平之间的关联。
我们分析了体重正常以及超重或肥胖青少年的UCP1 -3826 C/T、UCP2-866 G/A、Ala55Val和UCP3 -55 C/T基因多态性以及脂肪因子水平。该研究纳入了270名年龄在12至18岁之间的学生，这些学生根据墨西哥城的百分位数进行分类。采用免疫分析方法测量脂肪因子水平，并使用Taqman实时聚合酶链反应（RT-PCR）确定UCP基因多态性。
在所研究的七种遗传模型中，UCP基因多态性未发现显著差异。临床参数的大多数显著差异是在隐性模型下发现的，UCP2 -866多态性与舒张压（p=0.008）、甘油三酯（p=0.045）、低密度脂蛋白胆固醇（LDL-C）（p=0.003）、高密度脂蛋白胆固醇（HDL-C）（p=0.050）和瘦素血浆水平（p<0.001）相关。此外，在GA+AA与GG（隐性模型）比较中，肥胖组的瘦素水平较高，脂联素水平较低。
本研究在隐性模型中证明了临床特征与UCP2-866之间的直接关系，这与墨西哥肥胖或超重青少年人群中瘦素水平升高和脂联素水平降低有关。
