Nanomedicine & Nanobiotechnology Lab, Department of Biosciences, Integral University, Lucknow 226026, India.
Department of Physics (Nanoscience), Integral University, Lucknow 226026, India.
Microb Pathog. 2018 Jun;119:42-48. doi: 10.1016/j.micpath.2018.04.011. Epub 2018 Apr 7.
In this study, monodispersed, highly biocompatible and substantially stable glucose encapsulated CdO nanoparticles (G-CdO NPs) of uniform sizes were synthesized using a sol-gel route. In addition, naked CdO (n-CdO) NPs without any capping or surface functionalization were synthesized using the same method. These NPs were uniformly dispersed in an aqueous solution. The synthesis of G-CdO and n-CdO NP was confirmed by UV-Vis spectroscopy, transmission electron microscopy (TEM), zeta potential, and dynamic light scattering analyses. The average size of G-CdO and n-CdO NP was found to be 17±1and 27 ± 1 nm, under TEM, respectively. X-ray diffraction analysis of G-CdO and n-CdO NPs confirmed their sizes to be 18.83 and 28.41 nm, respectively, and revealed their cubic crystal structures with no impurity. The surface functionalization of G-CdO NPs with glucose was confirmed by Nuclear Magnetic Resonance and Fourier-transform infrared spectroscopy analyses. As per our knowledge, this is the first report to investigate the potencies of G-CdO and n-CdO NPs against gram-negative and gram-positive multi-drug resistant (MDR) bacteria. The minimum inhibitory concentrations of G-CdO and n-CdO NPs were6.42 and 16.29 μg/ml, respectively, against Escherichia coli (NCIM 2571-MDR), whereas 7.5 μg/ml & 11.6 μg/ml, respectively against S. aureus (NCIM- 2079) as determined by the double dilution method. The minimum bactericidal concentration was determined at the concentration for which no growth was observed. TEM analysis of E. coli cells treated with G-CdO NPs revealed cell shrinkage and degraded cell membranes, while the cell surfaces of untreated viable cells were smooth.
在这项研究中，使用溶胶-凝胶法合成了单分散、高生物相容性和高度稳定的葡萄糖包封的 CdO 纳米粒子（G-CdO NPs）。此外，还使用相同的方法合成了没有任何封端或表面功能化的裸 CdO（n-CdO）纳米粒子。这些纳米粒子均匀分散在水溶液中。通过紫外-可见光谱、透射电子显微镜（TEM）、Zeta 电位和动态光散射分析证实了 G-CdO 和 n-CdO NP 的合成。TEM 下 G-CdO 和 n-CdO NP 的平均粒径分别为 17±1 和 27±1nm。G-CdO 和 n-CdO NPs 的 X 射线衍射分析证实其尺寸分别为 18.83nm 和 28.41nm，均为立方晶体结构，无杂质。通过核磁共振和傅里叶变换红外光谱分析证实了 G-CdO NPs 的葡萄糖表面功能化。据我们所知，这是首次研究 G-CdO 和 n-CdO NPs 对革兰氏阴性和革兰氏阳性多药耐药（MDR）细菌的潜力。通过双稀释法测定，G-CdO 和 n-CdO NPs 对大肠杆菌（NCIM 2571-MDR）的最小抑菌浓度分别为 6.42μg/ml 和 16.29μg/ml，而对金黄色葡萄球菌（NCIM-2079）的最小抑菌浓度分别为 7.5μg/ml 和 11.6μg/ml。最低杀菌浓度是在没有观察到生长的浓度下确定的。用 TEM 分析用 G-CdO NPs 处理的大肠杆菌细胞，发现细胞收缩和细胞膜降解，而未经处理的活细胞表面光滑。
