Research Center, The Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050017, People's Republic of China; Tumor Research Institute, The Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050017, People's Republic of China.
Research Center, The Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050017, People's Republic of China.
Cancer Lett. 2018 Jul 10;426:37-46. doi: 10.1016/j.canlet.2018.03.049. Epub 2018 Apr 7.
As the most well-known circular RNA, ciRS-7 (also termed CDR1as) has been reported to act as a miR-7 sponge, resulting in reduced miR-7 activity and increased miR-7-targeted transcripts. Here, we showed that ciRS-7 is up-regulated in esophageal squamous cell carcinoma (ESCC), and is associated with the poor clinicopathological parameters of ESCC patients. Moreover, over-expression of ciRS-7 increased the proliferation, migration and invasion of ESCC cells. Mechanistic studies revealed that ciRS-7 contains nineteen miR-876-5p binding sites and acts as a miR-876-5p sponge. Over-expression of ciRS-7 resulted in the reduced tumor-repressive function of miR-876-5p on its downstream target MAGE-A family. In animal experiments, enforced ciRS-7 increased ESCC tumor growth and metastasis through targeting miR-876-5p/MAGE-A family axis. Collectively, our study provided novel evidence that ciRS-7 accelerates ESCC progression by acting as a miR-876-5p sponge to enhance MAGE-A family expression.
作为最著名的环状 RNA，ciRS-7（也称为 CDR1as）已被报道可作为 miR-7 的海绵，导致 miR-7 活性降低和 miR-7 靶向转录本增加。在这里，我们表明 ciRS-7 在食管鳞状细胞癌（ESCC）中上调，并与 ESCC 患者的不良临床病理参数相关。此外，ciRS-7 的过表达增加了 ESCC 细胞的增殖、迁移和侵袭。机制研究表明，ciRS-7 包含十九个 miR-876-5p 结合位点，并作为 miR-876-5p 的海绵。ciRS-7 的过表达导致 miR-876-5p 对其下游靶标 MAGE-A 家族的肿瘤抑制功能降低。在动物实验中，强制表达 ciRS-7 通过靶向 miR-876-5p/MAGE-A 家族轴增加 ESCC 肿瘤的生长和转移。总之，我们的研究提供了新的证据，表明 ciRS-7 通过作为 miR-876-5p 的海绵来增强 MAGE-A 家族的表达，从而加速 ESCC 的进展。
