Institute of Nano Biomedicine and Engineering, Shanghai Engineering Research Centre for Intelligent Diagnosis and Treatment Instrument, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan RD, Shanghai 200240, PR China.
School of Materials and Chemical Engineering, Chongqing University of Arts and Sciences, Yongchuan, Chongqing 402160, PR China.
Biomaterials. 2018 Jul;170:1-11. doi: 10.1016/j.biomaterials.2018.03.048. Epub 2018 Mar 28.
The cytotoxicity and unique tumor-tropic properties of cytokine-induced killer (CIK) cells render them promising in the field of cancer immunotherapy and delivery systems. Here, we report a novel and facile approach to assemble gold nanoclusters (GNCs) into stable and monodispersed nanoparticles (NPs) using Chlorin e6 (Ce6) molecules. Notably, the fluorescence intensity of the GNCs-Ce6 NPs was about 4.5 folds stronger than the GNCs counterparts. The as-prepared GNCs-Ce6 NPs were conjugated with CD3 antibody (Ab) and further employed to label CIK cells to create a CIK cell-based drug delivery system (Ce6-GNCs-Ab-CIK). The Ce6-GNCs-Ab-CIK exhibited high tumor-targeting efficiency and excellent therapeutic efficacy toward MGC-803 tumor-bearing mice. Benefiting from the synergistic therapeutic effect between GNCs-Ce6-Ab NPs and CIK cells, the GNCs-Ce6-Ab-CIK strategy may present an ideal cancer theranostic platform for tumor targeted imaging and combination therapy.
细胞因子诱导的杀伤（CIK）细胞的细胞毒性和独特的肿瘤趋向特性使它们在癌症免疫治疗和递药系统领域具有广阔的应用前景。在这里，我们报道了一种新颖且简便的方法，使用叶绿素 e6（Ce6）分子将金纳米簇（GNCs）组装成稳定且单分散的纳米颗粒（NPs）。值得注意的是，GNCs-Ce6 NPs 的荧光强度比 GNCs 本身要强约 4.5 倍。所制备的 GNCs-Ce6 NPs 与 CD3 抗体（Ab）偶联，进一步用于标记 CIK 细胞，以构建基于 CIK 细胞的药物递药系统（Ce6-GNCs-Ab-CIK）。Ce6-GNCs-Ab-CIK 对 MGC-803 荷瘤小鼠具有高肿瘤靶向效率和优异的治疗效果。得益于 GNCs-Ce6-Ab NPs 和 CIK 细胞之间的协同治疗效果，GNCs-Ce6-Ab-CIK 策略可能为肿瘤靶向成像和联合治疗提供一个理想的癌症治疗平台。
