产气肠杆菌代谢物促进铜绿微囊藻生物质回收以实现可持续生物絮凝剂和生物氢气生产。
Enterobacter aerogenes metabolites enhance Microcystis aeruginosa biomass recovery for sustainable bioflocculant and biohydrogen production.
机构信息
School of Environment, Northeast Normal University, Changchun 130117, China; Jilin Institute of Chemical Technology, Jilin, 132022, China; Key Laboratory for Vegetation Ecology, Ministry of Education, NO. 2555 Jingyue Street, Changchun 130117, China; State Environmental Protection Key Laboratory of Wetland Ecology and Vegetation Restoration, Northeast Normal University, Changchun, 130024, China.
School of Environment, Northeast Normal University, Changchun 130117, China; Key Laboratory for Vegetation Ecology, Ministry of Education, NO. 2555 Jingyue Street, Changchun 130117, China; State Environmental Protection Key Laboratory of Wetland Ecology and Vegetation Restoration, Northeast Normal University, Changchun, 130024, China.
出版信息
Sci Total Environ. 2018 Sep 1;634:488-496. doi: 10.1016/j.scitotenv.2018.03.327. Epub 2018 Apr 7.
We report a recycling bioresource involving harvesting of Microcystis aeruginosa using the bioflocculant (MBF-32) produced by Enterobacter aerogenes followed by the recovery of the harvested M. aeruginosa as the main substrate for the sustainable production of MBF-32 and biohydrogen. The experimental results indicate that the efficiency of bioflocculation exceeded 90% under optimal conditions. The harvested M. aeruginosa was further recycled as the main substrate for the supply of necessary elements. The highest yield (3.6±0.1g/L) of MBF-32 could be obtained from 20g/L of wet biomass of M. aeruginosa with an additional 20g/L of glucose as the extra carbon source. The highest yield of biohydrogen was 35mL of H/g (dw) algal biomass, obtained from 20g/L of wet biomass of M. aeruginosa with an additional 10g/L of glycerol. Transcriptome analyses indicated that MBF-32 was mainly composed of polysaccharide and tyrosine/tryptophan proteins. Furthermore, NADH synthase and polysaccharide export-related genes were found to be up-regulated.
我们报告了一种再利用生物资源的方法，涉及使用肠杆菌 Aerogenes 产生的生物絮凝剂 (MBF-32) 收获铜绿微囊藻，然后将收获的铜绿微囊藻作为可持续生产 MBF-32 和生物氢的主要基质进行回收。实验结果表明，在最佳条件下，生物絮凝的效率超过 90%。收获的铜绿微囊藻进一步循环利用，作为供应必要元素的主要基质。从 20g/L 的湿铜绿微囊藻生物质中，可获得最高产量（3.6±0.1g/L）的 MBF-32，外加 20g/L 的葡萄糖作为额外的碳源。从 20g/L 的湿铜绿微囊藻生物质中，添加 10g/L 的甘油，可获得最高产量的生物氢 35mL 的 H/g（dw）藻类生物质。转录组分析表明，MBF-32 主要由多糖和酪氨酸/色氨酸蛋白组成。此外，还发现 NADH 合酶和多糖出口相关基因上调。
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