Department of Chemical Engineering, Tsinghua University, Beijing 100084, PR China.
Bioresour Technol. 2011 Sep;102(18):8344-9. doi: 10.1016/j.biortech.2011.06.018. Epub 2011 Jun 13.
Enterobacter aerogenes is one of the most widely-studied model strains for fermentative hydrogen production. To improve the hydrogen yield of E. aerogenes, the bioengineering on a biomolecular level and metabolic network level is of importance. In this review, the fermentative technology of E. aerogenes for hydrogen production will be first briefly summarized. And then the bioengineering of E. aerogenes for the improvement of hydrogen yield will be thoroughly reviewed, including the anaerobic metabolic networks for hydrogen evolution in E. aerogenes, metabolic engineering for improving hydrogen production in E. aerogenes and mixed culture of E. aerogenes with other hydrogen-producing bacteria to enhance the overall yield in anaerobic cultivation. Finally, a perspective on E. aerogenes as a hydrogen producer including systems bioengineering approach for improving the hydrogen yield and application of the engineered E. aerogenes in mixed culture will be presented.
产气肠杆菌是发酵产氢研究最广泛的模式菌株之一。为了提高产气肠杆菌的产氢能力，在生物分子水平和代谢网络水平上进行生物工程改造非常重要。本文首先简要综述了产气肠杆菌发酵产氢的技术，然后深入综述了产气肠杆菌提高产氢能力的生物工程，包括产气肠杆菌中用于产氢的厌氧代谢网络、提高产气肠杆菌产氢能力的代谢工程以及与其他产氢细菌混合培养以提高厌氧培养中的整体产氢量。最后，对产气肠杆菌作为产氢菌进行了展望，包括提高产氢能力的系统生物工程方法以及工程化产气肠杆菌在混合培养中的应用。
