Miroshnichenko Igor I, Baymeeva Natalia V
Mental Health Research Center, Moscow, Russia.
J Chromatogr Sci. 2018 Jul 1;56(6):510-517. doi: 10.1093/chromsci/bmy024.
A quantitative method was developed to support therapeutic drug monitoring of eight antipsychotic drugs: chlorpromazine, haloperidol, zuclopenthixol, clozapine, risperidone, quetiapine, aripiprazole or olanzapine and some active metabolites (dehydroaripiprazole, N-desmethylclozapine and 9-hydroxyrisperidone) in human serum. Separation of the compounds was achieved using a Zorbax SB-C18 (150 mm × 4.6 mm, 5 μm) column and mass-spectrometric detection in multiple reaction monitoring mode. Human blood samples were collected in vacutainer tubes and the analytes were extracted with methyl-tert-butyl ether. The lower limits of quantitation were equal 0.5-1 ng/mL for all analytes. The method was applied with success to serum samples from schizophrenic patients undergoing polypharmacy with two or more different antipsychotic drugs. Precision data, accuracy results were satisfactory, and no interference from other psychotropic drugs was found. Hence, the method is suitable for the TDM of the analytes in psychotic patients' serum.
已开发出一种定量方法，用于支持对人血清中8种抗精神病药物（氯丙嗪、氟哌啶醇、珠氯噻醇、氯氮平、利培酮、喹硫平、阿立哌唑或奥氮平）以及一些活性代谢物（脱氢阿立哌唑、N-去甲基氯氮平和9-羟基利培酮）进行治疗药物监测。使用Zorbax SB-C18（150 mm×4.6 mm，5μm）色谱柱并采用多反应监测模式进行质谱检测，实现了化合物的分离。将人体血样采集到真空采血管中，并用甲基叔丁基醚萃取分析物。所有分析物的定量下限均为0.5-1 ng/mL。该方法已成功应用于接受两种或更多种不同抗精神病药物联合治疗的精神分裂症患者的血清样本。精密度数据、准确度结果均令人满意，且未发现其他精神药物的干扰。因此，该方法适用于精神病患者血清中分析物的治疗药物监测。
