Inserm U1026, University of Bordeaux, Tissue Bioengineering, U1026, F-33076 Bordeaux, France; CHU Bordeaux, Dentistry and Oral Health Department, F-33076 Bordeaux, France.
Inserm U1148, LVTS, X. Bichat Hospital, University Paris Diderot F-75018 Paris & Institut Galilée, University Paris 13, 93430 Villetaneuse, France.
Dent Mater. 2018 Jul;34(7):1024-1035. doi: 10.1016/j.dental.2018.03.021. Epub 2018 Apr 7.
Polysaccharide-based composite matrices consisting of natural polysaccharides, pullulan and dextran supplemented with hydroxyapatite (Matrix-HA) have recently been developed. The principal objective of this study was to evaluate the capacities of this composite material to promote new bone formation in a sinus lift model in the sheep. Secondary objectives were to evaluate in vitro properties of the material regarding cell adhesion and proliferation.
In this report, once such composite matrix was prepared as injectable beads after dispersion in a physiological buffer, and evaluated using a large animal model (sheep) for a sinus lift procedure.
In vitro studies revealed that these microbeads (250-550μm in diameter) allow vascular cell adhesion and proliferation of Endothelial Cells (EC) after 1 and 7 days of culture. In vivo studies were performed in 12 adult sheep, and newly formed tissue was analyzed by Cone Beam Computed Tomography (CBCT scanning electron microscopy (SEM) and by histology 3 and 6 months post-implantation. CBCT analyses at the implantation time revealed the radiolucent properties of these matrices. Quantitative analysis showed an increase of a dense mineralized tissue in the Matrix-HA group up to 3 months of implantation. The mineralized volume over total volume after 6 months reached comparable values to those obtained for Bio-Oss used as positive control. Histological examination confirmed that the Matrix-HA did not induce any long term inflammatory events, and promoted direct contact between the osteoid tissue and lamellar bone structures and beads. After 6 months, we observed a dense network of osteocytes surrounding both biomaterials as well as a newly vascularized formed tissue in close contact to the biomaterials.
In conclusion, the absence of animal components in Matrix-HA, the osteoconductive property of Matrix-HA in sheep, resulting in a dense bone and vascularized tissue, and the initial radiolucent property to follow graft integration offer great promises of this composite material for clinical use.
最近开发了一种由天然多糖、普鲁兰和右旋糖酐组成的多糖复合基质，并用羟基磷灰石（Matrix-HA）进行补充。本研究的主要目的是评估该复合材料在绵羊窦提升模型中促进新骨形成的能力。次要目标是评估该材料在细胞黏附和增殖方面的体外性能。
在本报告中，将该复合材料制备成可注射珠粒，分散在生理缓冲液中，然后使用大型动物模型（绵羊）进行窦提升手术进行评估。
体外研究表明，这些微球（直径 250-550μm）允许血管细胞黏附和内皮细胞（EC）在培养 1 天和 7 天后增殖。在 12 只成年绵羊中进行了体内研究，通过锥形束 CT（CBCT）扫描电子显微镜（SEM）和植入后 3 个月和 6 个月的组织学分析新形成的组织。植入时的 CBCT 分析显示出这些基质的透光特性。定量分析显示，在植入 3 个月时，Matrix-HA 组的致密矿化组织增加。植入 6 个月后，矿化体积与总体积之比达到与作为阳性对照的 Bio-Oss 获得的可比值。组织学检查证实 Matrix-HA 不会引起任何长期炎症事件，并促进类骨质组织与板层骨结构和微球之间的直接接触。6 个月后，我们观察到围绕两种生物材料形成的密集的骨细胞网络，以及与生物材料紧密接触的新血管化形成的组织。
Matrix-HA 中不含动物成分、Matrix-HA 在绵羊中的骨诱导特性导致致密的骨和血管化组织、以及初始透光特性以跟踪移植物整合，这为该复合材料的临床应用提供了很大的前景。
