Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, 701 West 168th St #1120, New York, NY, 10032, USA.
Department of Biology, University of Konstanz, Universitätsstrasse, 10, Box M643, 78467, Konstanz, Germany.
Sci Rep. 2018 Apr 10;8(1):5741. doi: 10.1038/s41598-018-24053-3.
In Planar cell polarity (PCP), cells coordinately polarize their cytoskeletons within the plane of the epithelium in which they lie. In most insect epithelia this is indicated by the coordinated projections of the hairs secreted by the ectodermal cells. PCP of this form has been effectively studied in Drosophila, but it has proven difficult to achieve an integrated description of the roles played by the various proteins. In the insect eye, PCP is not evident as the polarization of individual cells, but as the asymmetric arrangements of the cells of the ommatidia. This different form of PCP allows different studies to be performed, and using this system we have detected the action of two antagonistic signaling pathways. Even though antagonistic, the two pathways synergize and cooperate to ensure that the correct arrangement of the cells is achieved. The cooperative use of antagonistic signaling pathways occurs in the polarization of chemotacting cells, and we discuss the possibility that a similar molecular principle may underlie PCP.
在平面细胞极性 (PCP) 中，细胞在其所在的上皮层的平面内协调地极化其细胞骨架。在大多数昆虫上皮中，这表现为外胚层细胞分泌的毛发的协调突起。这种形式的 PCP 在果蝇中得到了有效研究，但要对各种蛋白质所扮演的角色进行综合描述，却一直很困难。在昆虫的眼睛中，PCP 并不是通过单个细胞的极化来表现，而是通过小眼的细胞的不对称排列来表现。这种不同形式的 PCP 允许进行不同的研究，我们使用这个系统检测到了两种拮抗信号通路的作用。尽管是拮抗的，但这两种通路协同合作，以确保实现细胞的正确排列。拮抗信号通路的协同使用发生在趋化细胞的极化过程中，我们讨论了类似的分子原理是否可能是 PCP 的基础。
